Chapter title |
The Sister Chromatid Exchange (SCE) Assay.
|
---|---|
Chapter number | 32 |
Book title |
Molecular Toxicology Protocols
|
Published in |
Methods in molecular biology, March 2014
|
DOI | 10.1007/978-1-62703-739-6_32 |
Pubmed ID | |
Book ISBNs |
978-1-62703-738-9, 978-1-62703-739-6
|
Authors |
Stults DM, Killen MW, Pierce AJ, Dawn M. Stults, Michael W. Killen, Andrew J. Pierce |
Editors |
Phouthone Keohavong, Stephen G. Grant |
Abstract |
A fully optimized staining method for detecting sister chromatid exchanges in cultured cells is presented. The method gives reproducibly robust quantitative results. Sister chromatid exchange is a classic toxicology assay for genotoxicity and for detecting alterations to the biochemistry underlying cellular homologous recombination. Growth of cells in the presence of 5'-bromo-deoxyuridine for two rounds of DNA replication followed by collecting metaphase spreads on glass slides, treatment with the UV-sensitive dye Hoechst 33258, long-wave UV light exposure, and Giemsa staining gives a permanent record of the exchanges. |
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