Chapter title |
Analysis of protein changes using two-dimensional difference gel electrophoresis.
|
---|---|
Chapter number | 2 |
Book title |
Molecular Toxicology Protocols
|
Published in |
Methods in molecular biology, March 2014
|
DOI | 10.1007/978-1-62703-739-6_2 |
Pubmed ID | |
Book ISBNs |
978-1-62703-738-9, 978-1-62703-739-6
|
Authors |
Gao W, Weimin Gao |
Editors |
Phouthone Keohavong, Stephen G. Grant |
Abstract |
A protocol for protein analysis using two-dimensional difference gel electrophoresis (2D-DIGE) is described. 2D-DIGE is one of the most popular and versatile methods of protein separation among rapidly increasing proteomics technologies. Similar to traditional two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), the proteins are separated based on their charges and molecular weight by 2D-DIGE. Different from 2D-PAGE, proteins are pre-labeled with different fluorescent and different protein samples are run in one gel by this method. Therefore, 2D-DIGE not only carries the advantages of 2D-PAGE but also eliminates gel-to-gel variation and achieves high resolution, sensitivity, and reproducibility. |
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