Chapter title |
Quantitative Method of Measuring Phosphatidylserine Externalization During Apoptosis Using Electron Paramagnetic Resonance (EPR) Spectroscopy and Annexin-Conjugated Iron.
|
---|---|
Chapter number | 42 |
Book title |
Molecular Toxicology Protocols
|
Published in |
Methods in molecular biology, March 2014
|
DOI | 10.1007/978-1-62703-739-6_42 |
Pubmed ID | |
Book ISBNs |
978-1-62703-738-9, 978-1-62703-739-6
|
Authors |
Fabisiak JP, Borisenko GG, Kagan VE, James P. Fabisiak, Grigory G. Borisenko, Valerian E. Kagan |
Editors |
Phouthone Keohavong, Stephen G. Grant |
Abstract |
We present here the application of a novel assay that measures the absolute amount of PS externalized on the surface of cells. While based on the same annexin binding principle as the fluorescent flow cytometry assay, we use paramagnetic iron as the ultimate reporter molecule, establishing a linear relationship between signal amplitude and amount of PS on the cell surface, allowing a quantitative assay of PS externalization over a wide dynamic range. The application of this technique, alone and in concert with the PS oxidation method presented in the previous chapter, will greatly aid in studying the mechanistic connection between lipid peroxidation and translocation events during apoptosis. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 36% |
Student > Bachelor | 2 | 14% |
Professor | 2 | 14% |
Researcher | 2 | 14% |
Student > Master | 1 | 7% |
Other | 1 | 7% |
Unknown | 1 | 7% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 29% |
Medicine and Dentistry | 3 | 21% |
Agricultural and Biological Sciences | 1 | 7% |
Immunology and Microbiology | 1 | 7% |
Chemistry | 1 | 7% |
Other | 0 | 0% |
Unknown | 4 | 29% |