Chapter title |
Investigating Cellular Quiescence of T Lymphocytes and Antigen-Induced Exit from Quiescence
|
---|---|
Chapter number | 12 |
Book title |
Cellular Quiescence
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7371-2_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7370-5, 978-1-4939-7371-2
|
Authors |
Kai Yang, Hongbo Chi |
Abstract |
Naïve T cells are in a quiescent state under homeostasis but respond to antigen stimulation by exiting from quiescence and entering the cell cycle. Appropriate regulation of quiescence is crucial for maintaining T cell homeostasis at steady state and initiating proper T cell responses to antigen stimulation. Emerging evidence indicates that signaling by mechanistic target of rapamycin (mTOR) plays a central role in the control of T cell quiescence and antigen-induced exit from quiescence through coordinating immune signals, cellular metabolic programs, and cell cycle machinery. The mTOR-dependent regulation of quiescence has also been implicated in the differentiation and function of memory T cells. In this chapter, we describe techniques to assess quiescent state of naïve T cells under steady state and exit from quiescence upon TCR stimulation. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Unspecified | 1 | 14% |
Researcher | 1 | 14% |
Student > Doctoral Student | 1 | 14% |
Unknown | 4 | 57% |
Readers by discipline | Count | As % |
---|---|---|
Unspecified | 1 | 14% |
Biochemistry, Genetics and Molecular Biology | 1 | 14% |
Unknown | 5 | 71% |