Chapter title |
Analysis of Murine Hematopoietic Stem Cell Proliferation During Inflammation
|
---|---|
Chapter number | 14 |
Book title |
Cellular Quiescence
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7371-2_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7370-5, 978-1-4939-7371-2
|
Authors |
Emilie Jalbert, Eric M. Pietras |
Abstract |
Normally, quiescent hematopoietic stem cells (HSC) rapidly enter the cell cycle following exposure to inflammatory stimuli. The analysis of HSC cell cycle activity in murine bone marrow during inflammation is often complicated by the relative rarity of HSCs and shifts in Sca-1, a key cell surface marker used to identify HSCs. Here, we report a method to analyze HSC proliferation and cell cycle distribution under inflammatory conditions. Our approach uses EdU incorporation and Ki67 staining coupled with DNA content quantification by DAPI. We also incorporate the surface marker ESAM to help minimize the potential for contaminating events that may confound analysis in the HSC compartment. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor | 2 | 20% |
Student > Bachelor | 2 | 20% |
Unspecified | 1 | 10% |
Student > Doctoral Student | 1 | 10% |
Student > Master | 1 | 10% |
Other | 1 | 10% |
Unknown | 2 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 3 | 30% |
Agricultural and Biological Sciences | 2 | 20% |
Nursing and Health Professions | 1 | 10% |
Immunology and Microbiology | 1 | 10% |
Unspecified | 1 | 10% |
Other | 0 | 0% |
Unknown | 2 | 20% |