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X Demographics
Mendeley readers
Attention Score in Context
| Chapter title |
In Vitro Translation-Based Protein Kinase Substrate Identification
|
|---|---|
| Chapter number | 15 |
| Book title |
Cell-Free Protein Synthesis
|
| Published in |
Methods in molecular biology, December 2013
|
| DOI | 10.1007/978-1-62703-782-2_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-62703-781-5, 978-1-62703-782-2
|
| Authors |
Szilvia K. Nagy, Tamás Mészáros |
| Editors |
Kirill Alexandrov, Wayne A. Johnston |
| Abstract |
Identification of a particular protein as a physiological substrate towards kinases of interest is an extremely complex process. Under physiological conditions kinases and their putative substrates are in low abundance, and production of active eukaryotic kinases with standard overexpressing methods is an arduous task. Herein, we describe a cell-free in vitro protein translation procedure combined with fluorescent phosphoprotein staining as a simple and rapid method for identification of putative kinase substrates. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 13 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 4 | 31% |
| Professor > Associate Professor | 2 | 15% |
| Student > Ph. D. Student | 1 | 8% |
| Student > Bachelor | 1 | 8% |
| Researcher | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 3 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 6 | 46% |
| Biochemistry, Genetics and Molecular Biology | 2 | 15% |
| Medicine and Dentistry | 1 | 8% |
| Unknown | 4 | 31% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 September 2014.
All research outputs
#18,378,085
of 22,763,032 outputs
Outputs from Methods in molecular biology
#7,870
of 13,088 outputs
Outputs of similar age
#230,672
of 306,065 outputs
Outputs of similar age from Methods in molecular biology
#286
of 575 outputs
Altmetric has tracked 22,763,032 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,088 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 306,065 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 13th percentile – i.e., 13% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 575 others from the same source and published within six weeks on either side of this one. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.