Cell-Free Protein Synthesis

Chapter 1 Production of Eukaryotic Cell-Free Lysate from Leishmania tarentolae.

Chapter 2 Bioinformatics analysis and optimization of cell-free protein synthesis.

Chapter 3 A cell-free expression screen to identify fusion tags for improved protein expression.

Chapter 4 One-pot, microscale cell-free enzyme expression and screening.

Chapter 5 Cell-free translation of biofuel enzymes.

Chapter 6 Cloning-independent expression and screening of enzymes using cell-free protein synthesis systems.

Chapter 7 High-level cell-free production of membrane proteins with nanodiscs.

Chapter 8 Cell-free protein-based enzyme discovery and protein-ligand interaction study.

Chapter 9 Human cell extract-derived cell-free systems for virus synthesis.

Chapter 10 Cell-free protein synthesis in microfluidic 96-well plates.

Chapter 11 Preparation of Multiple Site-Specific Mutant Proteins for NMR Studies by PCR-Directed Cell-Free Protein Synthesis.

Chapter 12 Site-specific incorporation of unnatural amino acids into proteins by cell-free protein synthesis.

Chapter 13 In Vitro Translation of Papillomavirus Authentic and Codon-Modified L1 Capsid Gene mRNAs in Mouse Keratinocyte Cell-Free Lysate.

Chapter 14 An optimized yeast cell-free lysate system for in vitro translation of human virus mRNA.

Chapter 15 In Vitro Translation-Based Protein Kinase Substrate Identification

Chapter 16 Preparation of Protein Arrays Using Cell-Free Protein Expression

Chapter 17 Posttranscriptional control of protein synthesis in Drosophila s2 cell-free system.

Chapter 18 Cell-free membrane protein expression.

Chapter 19 The PURE System for Protein Production.

Chapter 20 A cell-free protein synthesis system from insect cells.

Chapter 21 A cell-free expression platform for production of protein microarrays.