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Cell-Free Protein Synthesis

Overview of attention for book
Cover of 'Cell-Free Protein Synthesis'

Table of Contents

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    Book Overview
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    Chapter 1 Production of Eukaryotic Cell-Free Lysate from Leishmania tarentolae.
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    Chapter 2 Bioinformatics analysis and optimization of cell-free protein synthesis.
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    Chapter 3 A cell-free expression screen to identify fusion tags for improved protein expression.
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    Chapter 4 One-pot, microscale cell-free enzyme expression and screening.
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    Chapter 5 Cell-free translation of biofuel enzymes.
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    Chapter 6 Cloning-independent expression and screening of enzymes using cell-free protein synthesis systems.
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    Chapter 7 High-level cell-free production of membrane proteins with nanodiscs.
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    Chapter 8 Cell-free protein-based enzyme discovery and protein-ligand interaction study.
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    Chapter 9 Human cell extract-derived cell-free systems for virus synthesis.
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    Chapter 10 Cell-free protein synthesis in microfluidic 96-well plates.
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    Chapter 11 Preparation of Multiple Site-Specific Mutant Proteins for NMR Studies by PCR-Directed Cell-Free Protein Synthesis.
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    Chapter 12 Site-specific incorporation of unnatural amino acids into proteins by cell-free protein synthesis.
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    Chapter 13 In Vitro Translation of Papillomavirus Authentic and Codon-Modified L1 Capsid Gene mRNAs in Mouse Keratinocyte Cell-Free Lysate.
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    Chapter 14 An optimized yeast cell-free lysate system for in vitro translation of human virus mRNA.
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    Chapter 15 In Vitro Translation-Based Protein Kinase Substrate Identification
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    Chapter 16 Preparation of Protein Arrays Using Cell-Free Protein Expression
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    Chapter 17 Posttranscriptional control of protein synthesis in Drosophila s2 cell-free system.
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    Chapter 18 Cell-free membrane protein expression.
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    Chapter 19 The PURE System for Protein Production.
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    Chapter 20 A cell-free protein synthesis system from insect cells.
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    Chapter 21 A cell-free expression platform for production of protein microarrays.
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    Chapter 22 ERRATA
Attention for Chapter 4: One-pot, microscale cell-free enzyme expression and screening.
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age and source (55th percentile)

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Chapter title
One-pot, microscale cell-free enzyme expression and screening.
Chapter number 4
Book title
Cell-Free Protein Synthesis
Published in
Methods in molecular biology, December 2013
DOI 10.1007/978-1-62703-782-2_4
Pubmed ID
24395409
Book ISBNs
978-1-62703-781-5, 978-1-62703-782-2
Authors

Aarthi Chandrasekaran, Anup K Singh, Anup K. Singh, Chandrasekaran, Aarthi, Singh, Anup K.

Editors

Kirill Alexandrov, Wayne A. Johnston

Abstract

The lack of high-throughput approaches for expression and screening of large enzyme libraries remains a major bottleneck for current enzyme engineering efforts. To address this need, we have developed a high-throughput, fluorescence-based approach for rapid one-pot, microscale expression, and screening of industrial enzymes. In this chapter, we present the protocol for integration of cell-free protein expression with activity screening of enzymes in two formats: (1) a 96-well plate format and (2) a microscale-array format. Our one-pot method is ideally suited for rapid, first pass screening of enzymes and can also be used to perform detailed mechanistic analysis such as measurement of kinetics, determination of optimum temperature, and to study enzyme inhibition.

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X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 33%
Professor 1 17%
Researcher 1 17%
Student > Ph. D. Student 1 17%
Unknown 1 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 33%
Biochemistry, Genetics and Molecular Biology 2 33%
Chemical Engineering 1 17%
Unknown 1 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 January 2014.
All research outputs
#15,289,831
of 22,738,543 outputs
Outputs from Methods in molecular biology
#5,308
of 13,087 outputs
Outputs of similar age
#191,271
of 305,972 outputs
Outputs of similar age from Methods in molecular biology
#195
of 572 outputs
Altmetric has tracked 22,738,543 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,087 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 305,972 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 572 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 55% of its contemporaries.

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