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Cell-Free Protein Synthesis

Overview of attention for book
Cover of 'Cell-Free Protein Synthesis'

Table of Contents

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    Book Overview
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    Chapter 1 Production of Eukaryotic Cell-Free Lysate from Leishmania tarentolae.
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    Chapter 2 Bioinformatics analysis and optimization of cell-free protein synthesis.
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    Chapter 3 A cell-free expression screen to identify fusion tags for improved protein expression.
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    Chapter 4 One-pot, microscale cell-free enzyme expression and screening.
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    Chapter 5 Cell-free translation of biofuel enzymes.
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    Chapter 6 Cloning-independent expression and screening of enzymes using cell-free protein synthesis systems.
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    Chapter 7 High-level cell-free production of membrane proteins with nanodiscs.
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    Chapter 8 Cell-free protein-based enzyme discovery and protein-ligand interaction study.
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    Chapter 9 Human cell extract-derived cell-free systems for virus synthesis.
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    Chapter 10 Cell-free protein synthesis in microfluidic 96-well plates.
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    Chapter 11 Preparation of Multiple Site-Specific Mutant Proteins for NMR Studies by PCR-Directed Cell-Free Protein Synthesis.
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    Chapter 12 Site-specific incorporation of unnatural amino acids into proteins by cell-free protein synthesis.
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    Chapter 13 In Vitro Translation of Papillomavirus Authentic and Codon-Modified L1 Capsid Gene mRNAs in Mouse Keratinocyte Cell-Free Lysate.
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    Chapter 14 An optimized yeast cell-free lysate system for in vitro translation of human virus mRNA.
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    Chapter 15 In Vitro Translation-Based Protein Kinase Substrate Identification
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    Chapter 16 Preparation of Protein Arrays Using Cell-Free Protein Expression
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    Chapter 17 Posttranscriptional control of protein synthesis in Drosophila s2 cell-free system.
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    Chapter 18 Cell-free membrane protein expression.
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    Chapter 19 The PURE System for Protein Production.
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    Chapter 20 A cell-free protein synthesis system from insect cells.
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    Chapter 21 A cell-free expression platform for production of protein microarrays.
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    Chapter 22 ERRATA
Attention for Chapter 17: Posttranscriptional control of protein synthesis in Drosophila s2 cell-free system.
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Chapter title
Posttranscriptional control of protein synthesis in Drosophila s2 cell-free system.
Chapter number 17
Book title
Cell-Free Protein Synthesis
Published in
Methods in molecular biology, December 2013
DOI 10.1007/978-1-62703-782-2_17
Pubmed ID
Book ISBNs
978-1-62703-781-5, 978-1-62703-782-2
Authors

Motoaki Wakiyama, Shigeyuki Yokoyama, Wakiyama, Motoaki, Yokoyama, Shigeyuki

Editors

Kirill Alexandrov, Wayne A. Johnston

Abstract

Protein synthesis is regulated transcriptionally and posttranscriptionally, with the latter including both the translation and mRNA degradation steps. Eukaryotic mRNAs have a characteristic 7-methyl-G cap structure at their 5' ends and a polyadenylated tail at their 3' ends. These structures, and the sequences of the untranslated regions (UTR) flanking the coding region on the 5' and 3' sides, are recognized by various RNA-binding proteins and determine translational efficiency and mRNA stability. RNA interference is a sequence-specific inhibition of protein synthesis triggered by double-stranded RNA (dsRNA). This process is mediated by RNA-binding proteins named Argonaute. Argonautes incorporate dsRNAs of 21-22 nucleotides (termed short-interfering RNAs or siRNAs) and cleave mRNAs containing sequences complementary to siRNAs. In this chapter, we describe a cell-free translation system from Drosophila Schneider line 2 (S2) cells that recapitulates RNA interference. This system can be programmed with multiple RNA transcripts, a target and a control, and chemically synthesized short-interfering RNA (siRNA). The production of the target protein is reduced in the presence of the target-specific siRNA, in a dose-dependent manner. We also describe a coupled transcription and translation system using the S2 cell lysate.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 38%
Student > Ph. D. Student 2 25%
Professor 1 13%
Student > Master 1 13%
Unknown 1 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 50%
Biochemistry, Genetics and Molecular Biology 2 25%
Unknown 2 25%