Chapter title |
Probing Collagen Organization: Practical Guide for Second-Harmonic Generation (SHG) Imaging
|
---|---|
Chapter number | 27 |
Book title |
Fibrosis
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7113-8_27 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7112-1, 978-1-4939-7113-8
|
Authors |
Riccardo Cicchi, Francesco S. Pavone |
Abstract |
Second-harmonic generation (SHG) microscopy is a powerful microscopy technique for imaging collagen and other biological molecules using a label-free approach. SHG microscopy offers the advantages of a nonlinear imaging modality together with those ones of a coherent technique. These features make SHG microscopy the ideal tool for imaging collagen at high resolution and for characterizing its organization at various hierarchical levels. Considering that collagen organization plays a crucial role in fibrosis and in its development, it would be beneficial for the researcher working in the field of fibrosis to have a manual listing crucial points to be considered when imaging collagen using SHG microscopy. This chapter provides an answer to this demand with state-of-the-art protocols, methods, and laboratory tips related to SHG microscopy. We also discuss advantages and limitations of the use of SHG for studying fibrosis. |
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Student > Bachelor | 3 | 14% |
Researcher | 3 | 14% |
Student > Master | 2 | 9% |
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Other | 2 | 9% |
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Physics and Astronomy | 1 | 5% |
Other | 1 | 5% |
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