Chapter title |
Purification of Human Plasma/Cellular Fibronectin and Fibronectin Fragments
|
---|---|
Chapter number | 20 |
Book title |
Fibrosis
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7113-8_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7112-1, 978-1-4939-7113-8
|
Authors |
Giampiero Pietrocola, Simonetta Rindi, Giulia Nobile, Pietro Speziale |
Abstract |
A method is described for the purification of plasma fibronectins based on a combination of gelatin- and arginine-Sepharose chromatography steps. Cellular fibronectin can be purified from an osteosarcoma fibroblast cell line by affinity chromatography using a monoclonal antibody anti-fibronectin as ligand. Furthermore, we also provide a protocol for the purification of fibronectin domains obtained by fractionation of thermolysin-digested plasma fibronectin on ion-exchange/gel filtration chromatography columns. Assessment of the fibronectin purity is performed by SDS-PAGE, while the ligand binding activities of specific fibronectin domains are determined by ELISA. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 2 | 17% |
Student > Postgraduate | 2 | 17% |
Researcher | 2 | 17% |
Student > Ph. D. Student | 1 | 8% |
Professor | 1 | 8% |
Other | 1 | 8% |
Unknown | 3 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 42% |
Agricultural and Biological Sciences | 1 | 8% |
Psychology | 1 | 8% |
Engineering | 1 | 8% |
Unknown | 4 | 33% |