Report for: Extracellular Vesicles

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Chapter title	An Adaptable Polyethylene Glycol-Based Workflow for Proteomic Analysis of Extracellar Vesicles
Chapter number	25
Book title	Extracellular Vesicles
Published in	Methods in molecular biology, January 2017
DOI	10.1007/978-1-4939-7253-1_25
Pubmed ID	28828667
Book ISBNs	978-1-4939-7251-7, 978-1-4939-7253-1
Authors	Stephanie N. Hurwitz, David G. MeckesJr., David G. Meckes, Hurwitz, Stephanie N., Meckes, David G.
Abstract	Extracellular vesicles (EVs), including exosomes are endocytically derived nanovesicles expelled from cells that contain molecular information in the form of lipids, proteins, and nucleic acids. Transfer of this information to other cells in local or distant microenvironments facilitates cell-to-cell communication. Importantly, diseased cells release exosomes containing specific cargo that may contribute to pathology and can be harnessed for diagnostic or prognostic use. The broad potential medical utility of exosomes has fueled rapidly expanding research on understanding the composition and functions of exosomes in normal and pathological conditions. Here, we provide a complete workflow for purifying exosome-sized vesicles from biological fluids for in-depth proteomic analyses. Moreover, this polyethylene glycol-based method is efficient, highly adaptable, and compatible with a variety of downstream applications.

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Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country	Count	As %
Unknown	17	100%

Demographic breakdown

Readers by professional status	Count	As %
Student > Ph. D. Student	4	24%
Student > Master	3	18%
Professor	1	6%
Unspecified	1	6%
Student > Doctoral Student	1	6%
Other	1	6%
Unknown	6	35%

Readers by discipline	Count	As %
Biochemistry, Genetics and Molecular Biology	4	24%
Engineering	3	18%
Unspecified	1	6%
Chemical Engineering	1	6%
Chemistry	1	6%
Other	1	6%
Unknown	6	35%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 June 2021.

All research outputs

#8,386,799

of 25,059,640 outputs

Outputs from Methods in molecular biology

#2,596

of 14,096 outputs

Outputs of similar age

#146,434

of 432,187 outputs

Outputs of similar age from Methods in molecular biology

#256

of 1,084 outputs

Altmetric has tracked 25,059,640 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.

So far Altmetric has tracked 14,096 research outputs from this source. They receive a mean Attention Score of 3.5. This one has done well, scoring higher than 75% of its peers.

Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 432,187 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.

We're also able to compare this research output to 1,084 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.

Extracellular Vesicles

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Attention Score in Context