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Chapter title |
Size Exclusion Chromatography: A Simple and Reliable Method for Exosome Purification
|
---|---|
Chapter number | 9 |
Book title |
Extracellular Vesicles
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7253-1_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7251-7, 978-1-4939-7253-1
|
Authors |
Richard Lobb, Andreas Möller |
Abstract |
Exosomes were originally described 29 years ago as a mechanism for the removal of redundant molecules from reticulocytes, nothing but a process of removing cellular trash. It is now however, abundantly clear that exosomes have a more significant biological role. However, there is currently limited information pertaining to efficient isolation procedures that can be used to isolate exosomes from both cell culture media, and clinical samples such as plasma. Here, we present a reliable and efficient procedure that can be utilized for the isolation of exosomes from various starting materials. |
Mendeley readers
The data shown below were compiled from readership statistics for 54 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 54 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 12 | 22% |
Student > Bachelor | 7 | 13% |
Researcher | 7 | 13% |
Student > Doctoral Student | 3 | 6% |
Professor | 3 | 6% |
Other | 7 | 13% |
Unknown | 15 | 28% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 11 | 20% |
Agricultural and Biological Sciences | 8 | 15% |
Medicine and Dentistry | 7 | 13% |
Neuroscience | 3 | 6% |
Engineering | 3 | 6% |
Other | 3 | 6% |
Unknown | 19 | 35% |