Chapter title |
An Integrated Double-Filtration Microfluidic Device for Detection of Extracellular Vesicles from Urine for Bladder Cancer Diagnosis
|
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Chapter number | 29 |
Book title |
Extracellular Vesicles
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7253-1_29 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7251-7, 978-1-4939-7253-1
|
Authors |
Li-Guo Liang, Ye-Feng Sheng, Sherry Zhou, Fatih Inci, Lanjuan Li, Utkan Demirci, ShuQi Wang |
Abstract |
Extracellular vesicles (EVs) are present in a variety of bodily fluids and they play an important role in cellular communications and signal transduction mechanisms. Studies have shown that the number of EVs and EV-associated biomarkers (i.e., proteins, nucleic acids and lipids) can be used to aid clinical diagnosis. Although ultracentrifugation is commonly used for EV isolation, it is not practical for clinical settings. Here, we developed an integrated double-filtration device that isolated and enriched EVs from urine, and subsequently detected/quantified EVs from urine via microchip ELISA. Results showed that the concentration of EVs was significantly elevated compared to healthy controls. Receiver operating characteristic analysis demonstrated that this integrated EV quantification device had a sensitivity of 81.3% at a specificity of 90% (16 bladder cancer patients and eight healthy controls). Thus, this integrated device shows great potential to supplement urine cytology for diagnosis of bladder cancer in point-of-care (POC) settings. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 35 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 6 | 17% |
Student > Ph. D. Student | 5 | 14% |
Student > Postgraduate | 3 | 9% |
Student > Bachelor | 2 | 6% |
Student > Doctoral Student | 2 | 6% |
Other | 7 | 20% |
Unknown | 10 | 29% |
Readers by discipline | Count | As % |
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Unspecified | 2 | 6% |
Chemical Engineering | 2 | 6% |
Other | 4 | 11% |
Unknown | 11 | 31% |