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Extracellular Vesicles

Overview of attention for book
Cover of 'Extracellular Vesicles'

Table of Contents

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    Book Overview
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    Chapter 1 Extracellular Vesicles: A Brief Overview and Its Role in Precision Medicine
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    Chapter 2 Red Blood Cells: A Source of Extracellular Vesicles
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    Chapter 3 Isolation of Extracellular Vesicles by Ultracentrifugation
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    Chapter 4 Sequential Filtration: A Gentle Method for the Isolation of Functional Extracellular Vesicles
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    Chapter 5 Paper-Based for Isolation of Extracellular Vesicles
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    Chapter 6 Filter-Based Extracellular Vesicle mRNA Isolation and High-Throughput Gene Expression Analysis
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    Chapter 7 Specific and Generic Isolation of Extracellular Vesicles with Magnetic Beads
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    Chapter 8 Polymer-Based Purification of Extracellular Vesicles
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    Chapter 9 Size Exclusion Chromatography: A Simple and Reliable Method for Exosome Purification
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    Chapter 10 Purification Protocols for Extracellular Vesicles
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    Chapter 11 Characterization of Extracellular Vesicles by Surface Plasmon Resonance
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    Chapter 12 Extracellular Vesicle Isolation and Analysis by Western Blotting
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    Chapter 13 Analysis of Extracellular Vesicles Using Fluorescence Nanoparticle Tracking Analysis
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    Chapter 14 Characterization of Extracellular Vesicles by Flow Cytometry
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    Chapter 15 Characterization of Extracellular Vesicles by Size-Exclusion High-Performance Liquid Chromatography (HPLC)
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    Chapter 16 Multi-Surface Antigen Staining of Larger Extracellular Vesicles
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    Chapter 17 Microcapillary Chip-Based Extracellular Vesicle Profiling System
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    Chapter 18 Detection and Characterization of Extracellular Vesicles by Transmission and Cryo-Transmission Electron Microscopy
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    Chapter 19 Imaging of Isolated Extracellular Vesicles Using Fluorescence Microscopy
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    Chapter 20 In Vivo Tracking of Extracellular Vesicles in Mice Using Fusion Protein Comprising Lactadherin and Gaussia Luciferase
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    Chapter 21 Tracking Extracellular Vesicles Delivery and RNA Translation Using Multiplexed Reporters
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    Chapter 22 Extraction and Analysis of Extracellular Vesicle-Associated miRNAs Following Antibody-Based Extracellular Vesicle Capture from Plasma Samples
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    Chapter 23 Extracellular Vesicle miRNA Detection Using Molecular Beacons
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    Chapter 24 Rapid Isolation of Extracellular Vesicles from Blood Plasma with Size-Exclusion Chromatography Followed by Mass Spectrometry-Based Proteomic Profiling
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    Chapter 25 An Adaptable Polyethylene Glycol-Based Workflow for Proteomic Analysis of Extracellar Vesicles
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    Chapter 26 Protocol for Exosome Isolation from Small Volume of Ovarian Follicular Fluid: Evaluation of Ultracentrifugation and Commercial Kits
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    Chapter 27 Isolation of Extracellular Vesicles in Saliva Using Density Gradient Ultracentrifugation
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    Chapter 28 Isolation of Extracellular Vesicles from Breast Milk
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    Chapter 29 An Integrated Double-Filtration Microfluidic Device for Detection of Extracellular Vesicles from Urine for Bladder Cancer Diagnosis
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    Chapter 30 Electric Field-Induced Disruption and Releasing Viable Content from Extracellular Vesicles
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    Chapter 31 Production and Characterization of Extracellular Vesicles in Malaria
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    Chapter 32 Isolation of Extracellular Vesicles from Stem Cells
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    Chapter 33 The Use of Peripheral Extracellular Vesicles for Identification of Molecular Biomarkers in a Solid Tumor Mouse Model
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    Chapter 34 Therapeutic Applications of Extracellular Vesicles: Perspectives from Newborn Medicine
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    Chapter 35 Therapeutic Use of Tumor Cell-Derived Extracellular Vesicles
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    Chapter 36 Erratum to: An Adaptable Polyethylene Glycol-Based Workflow for Proteomic Analysis of Extracellular Vesicles
Attention for Chapter 13: Analysis of Extracellular Vesicles Using Fluorescence Nanoparticle Tracking Analysis
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Chapter title
Analysis of Extracellular Vesicles Using Fluorescence Nanoparticle Tracking Analysis
Chapter number 13
Book title
Extracellular Vesicles
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7253-1_13
Pubmed ID
28828655
Book ISBNs
978-1-4939-7251-7, 978-1-4939-7253-1
Authors

Pauline Carnell-Morris, Dionne Tannetta, Agnieszka Siupa, Patrick Hole, Rebecca Dragovic, Carnell-Morris, Pauline, Tannetta, Dionne, Siupa, Agnieszka, Hole, Patrick, Dragovic, Rebecca

Abstract

Fluorescence nanoparticle tracking analysis (fl-NTA) allows for accurate sizing, counting, and phenotyping of extracellular vesicles (EV). Here, we present two protocols for the analysis of EVs using fl-NTA, highlighting the potential pitfalls and challenges. The first protocol utilizes CellMask Orange™ (CMO) as a general membrane marker to label EVs derived from plasma. The second protocol describes the use of a Qdot-conjugated antibody to identify syncytiotrophoblast (STB)-derived EVs. "Standard" preparations of STB-derived EVs enriched for either microvesicles (STBMV) or exosomes (STBEX), containing a known amount of EV positive for the STB specific antigen placental alkaline phosphatase (PLAP), were also used to optimize fl-NTA camera settings.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 86 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 86 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 15 17%
Student > Bachelor 12 14%
Student > Doctoral Student 11 13%
Researcher 9 10%
Student > Master 7 8%
Other 10 12%
Unknown 22 26%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 26 30%
Medicine and Dentistry 10 12%
Agricultural and Biological Sciences 4 5%
Chemistry 4 5%
Veterinary Science and Veterinary Medicine 3 3%
Other 14 16%
Unknown 25 29%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 May 2018.
All research outputs
#23,381,499
of 25,998,826 outputs
Outputs from Methods in molecular biology
#11,274
of 14,458 outputs
Outputs of similar age
#370,271
of 428,501 outputs
Outputs of similar age from Methods in molecular biology
#863
of 1,097 outputs
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So far Altmetric has tracked 14,458 research outputs from this source. They receive a mean Attention Score of 3.5. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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