| Chapter title |
Application of a CAGE Method to an Avian Development Study
|
|---|---|
| Chapter number | 6 |
| Book title |
Avian and Reptilian Developmental Biology
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7216-6_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7215-9, 978-1-4939-7216-6
|
| Authors |
Ruslan Deviatiiarov, Marina Lizio, Oleg Gusev |
| Abstract |
Cap analysis of gene expression (CAGE) is a convenient approach for genome-wide identification of promoter regions at single base-pair resolution level and accurate expression estimation of the corresponding transcripts. Depending on the initial biomaterial amount and sequencing technology, different computational pipelines for data processing are available, as well as variations of the CAGE protocol that improve sensitivity and accuracy. Therefore, this chapter elucidates the key steps of sample preparation, sequencing, and data analysis via an example of a promoter expression estimation study in chicken development. We also describe the applicability of this approach for studying other avian and reptilian species. |
X Demographics
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| Country | Count | As % |
|---|---|---|
| Bosnia and Herzegovina | 1 | 50% |
| United Kingdom | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 8 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 38% |
| Professor | 1 | 13% |
| Lecturer > Senior Lecturer | 1 | 13% |
| Student > Ph. D. Student | 1 | 13% |
| Student > Postgraduate | 1 | 13% |
| Other | 0 | 0% |
| Unknown | 1 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 50% |
| Agricultural and Biological Sciences | 2 | 25% |
| Computer Science | 1 | 13% |
| Unknown | 1 | 13% |