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Avian and Reptilian Developmental Biology

Overview of attention for book
Avian and Reptilian Developmental Biology
Humana Press

Table of Contents

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    Book Overview
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    Chapter 1 Some Thoughts on Experimental Design
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    Chapter 2 Comparative Genomics as a Foundation for Evo-Devo Studies in Birds
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    Chapter 3 A Step-by-Step Guide to Assemble a Reptilian Genome
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    Chapter 4 Genomic and Transcriptomic Analyses of Avian Sex Chromosomes and Sex-Linked Genes
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    Chapter 5 Systems Biology Analyses in Chicken: Workflow for Transcriptome and ChIP-Seq Analyses Using the Chicken Skin Paradigm
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    Chapter 6 Application of a CAGE Method to an Avian Development Study
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    Chapter 7 CRISPR/Cas9 in the Chicken Embryo
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    Chapter 8 Fluorescent Quail: A Transgenic Model System for the Dynamic Study of Avian Development
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    Chapter 9 Lentiviral-Mediated Transgenesis in Songbirds
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    Chapter 10 In Ovo Electroporation Methods in Chick Embryos
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    Chapter 11 Genetic Manipulation of the Avian Urogenital System Using In Ovo Electroporation
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    Chapter 12 Enhancer Analyses Using Chicken Embryo Electroporation
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    Chapter 13 Transgene Introduction into the Chick Limb Bud by Electroporation
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    Chapter 14 Chicken Induced Pluripotent Stem Cells: Establishment and Characterization
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    Chapter 15 Isolation and Characterization of Chicken Primordial Germ Cells and Their Application in Transgenesis
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    Chapter 16 Handling of Gametes for In Vitro Insemination in Birds
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    Chapter 17 In Vitro and Ex Ovo Culture of Reptilian and Avian Neural Progenitor Cells
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    Chapter 18 Lifting the Veil on Reptile Embryology: The Veiled Chameleon (Chamaeleo calyptratus) as a Model System to Study Reptilian Development
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    Chapter 19 Model Clades Versus Model Species: Anolis Lizards as an Integrative Model of Anatomical Evolution
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    Chapter 20 The Feather Model for Chemo- and Radiation Therapy-Induced Tissue Damage
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    Chapter 21 An Early Chick Embryo Culture Device for Extended Continuous Observation
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    Chapter 22 A Sensitive and Versatile In Situ Hybridization Protocol for Gene Expression Analysis in Developing Amniote Brains
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    Chapter 23 Somitogenesis and Axial Development in Reptiles
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    Chapter 24 MicroCT Imaging on Living Alligator Teeth Reveals Natural Tooth Cycling
Attention for Chapter 17: In Vitro and Ex Ovo Culture of Reptilian and Avian Neural Progenitor Cells
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Chapter title
In Vitro and Ex Ovo Culture of Reptilian and Avian Neural Progenitor Cells
Chapter number 17
Book title
Avian and Reptilian Developmental Biology
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7216-6_17
Pubmed ID
Book ISBNs
978-1-4939-7215-9, 978-1-4939-7216-6
Authors

Wataru Yamashita, Toyo Shimizu, Tadashi Nomura

Abstract

Reptiles and birds have been highlighted as excellent experimental models for the study of developmental biology; however, due to technical limitations in cellular analysis, dynamics of neural stem/progenitor cells of these animals remain unclear. In this chapter, we introduce the protocols for neurosphere culture and ex ovo embryonic culture of developing reptilian and avian embryos, which are modified from the method originally established for rodent embryos. Applications of these techniques provide powerful strategies for the study of comparative neural development of amniotes.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 3 43%
Researcher 1 14%
Student > Doctoral Student 1 14%
Unknown 2 29%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 29%
Agricultural and Biological Sciences 2 29%
Neuroscience 1 14%
Unknown 2 29%