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Mendeley readers
Chapter title |
An Early Chick Embryo Culture Device for Extended Continuous Observation
|
---|---|
Chapter number | 21 |
Book title |
Avian and Reptilian Developmental Biology
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-7216-6_21 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7215-9, 978-1-4939-7216-6
|
Authors |
Hans-Georg Sydow, Tobias Pieper, Christoph Viebahn, Nikoloz Tsikolia |
Abstract |
Appropriate mechanical tension of the vitelline membrane as the culture substrate for the early chick embryo is frequently reported to be required for successful in vitro development. Here we describe a modified device, made of anodized aluminum, for in vitro culture which we used for studies of left-right symmetry breaking with emphasis on morphology and gene expression as readouts. The technique allows for easy, high-throughput tissue handling and provides a suitable tension in a stable and easily reproducible manner proven to be suitable for correct molecular left-right patterning and heart looping after long-term culture. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 50% |
Lecturer | 1 | 17% |
Student > Master | 1 | 17% |
Unknown | 1 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 50% |
Agricultural and Biological Sciences | 1 | 17% |
Psychology | 1 | 17% |
Unknown | 1 | 17% |