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Mendeley readers
Chapter title |
Isolation and Characterization of Chicken Primordial Germ Cells and Their Application in Transgenesis
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Chapter number | 15 |
Book title |
Avian and Reptilian Developmental Biology
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-7216-6_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7215-9, 978-1-4939-7216-6
|
Authors |
Jae Yong Han, Bo Ram Lee |
Abstract |
Primordial germ cells (PGCs), precursors of functional gametes, offer great promise for the use of genetic resources and transgenesis in chickens. PGCs can be isolated from the developing embryo at diverse early stages and are subsequently expandable in vitro. In vitro proliferating chicken PGCs can facilitate the production of efficient germline chimeras and transgenic chickens. Here, we describe methods to isolate and characterize PGCs from chicken embryos and their application to transgenesis. |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Other | 2 | 15% |
Student > Bachelor | 2 | 15% |
Student > Ph. D. Student | 2 | 15% |
Researcher | 2 | 15% |
Student > Postgraduate | 1 | 8% |
Other | 0 | 0% |
Unknown | 4 | 31% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 4 | 31% |
Biochemistry, Genetics and Molecular Biology | 3 | 23% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 8% |
Engineering | 1 | 8% |
Unknown | 4 | 31% |