Chapter title |
RNA Amplification for Pseudogene Detection Using RNA-Seq.
|
---|---|
Chapter number | 9 |
Book title |
Pseudogenes
|
Published in |
Methods in molecular biology, April 2014
|
DOI | 10.1007/978-1-4939-0835-6_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-0834-9, 978-1-4939-0835-6
|
Authors |
Tsoi SC, Dyck MK, Stephen C. M. Tsoi Ph.D., Michael K. Dyck, Stephen C. M. Tsoi, Tsoi, Stephen C. M., Dyck, Michael K. |
Editors |
Laura Poliseno |
Abstract |
Transcriptomic research using microarrays and RNA-Sequencing (RNA-seq) is now possible starting from minute biological samples, such as clinical specimens or embryos, due to the development of highly sensitive and reproducible cDNA synthesis methods. Here, we describe a quick method of RNA amplification and double-stranded cDNA synthesis starting with 10 ng of high-quality total RNA extracted from porcine embryos. The final product (double-stranded DNA) is adequate for the detection by RNA-seq of protein-coding transcripts, as well as of all the other classes of noncoding RNAs, including pseudogenes. |
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