↓ Skip to main content
What is this page? Embed badge

Pseudogenes

Overview of attention for book
Cover of 'Pseudogenes'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Pseudogene Redux with New Biological Significance
  3. Altmetric Badge
    Chapter 2 Contribution of Pseudogenes to Sequence Diversity
  4. Altmetric Badge
    Chapter 3 Computational Methods for Pseudogene Annotation Based on Sequence Homology
  5. Altmetric Badge
    Chapter 4 Computational Methods of Identification of Pseudogenes Based on Functionality: Entropy and GC Content.
  6. Altmetric Badge
    Chapter 5 Methods of Identification of Pseudogenes Based on Functionality: Hybridization of 18S rRNA and mRNA During Translation
  7. Altmetric Badge
    Chapter 6 Whole-Genome Identification of Neutrally Evolving Pseudogenes Using the Evolutionary Measure dN/dS
  8. Altmetric Badge
    Chapter 7 Methods to study the occurrence and the evolution of pseudogenes through a phylogenetic approach.
  9. Altmetric Badge
    Chapter 8 Methods for Detecting Transcribed Pseudogenes: PCR on Regions of High Sequence Similarity Followed by Cloning and Sequencing
  10. Altmetric Badge
    Chapter 9 RNA Amplification for Pseudogene Detection Using RNA-Seq.
  11. Altmetric Badge
    Chapter 10 GENCODE Pseudogenes
  12. Altmetric Badge
    Chapter 11 Methods to Detect Transcribed Pseudogenes: RNA-Seq Discovery Allows Learning Through Features
  13. Altmetric Badge
    Chapter 12 Proteomics techniques for the detection of translated pseudogenes.
  14. Altmetric Badge
    Chapter 13 Pseudogenes as Competitive Endogenous RNAs: Target Prediction and Validation.
  15. Altmetric Badge
    Chapter 14 Pseudogenes: A Novel Source of trans-Acting Antisense RNAs
  16. Altmetric Badge
    Chapter 15 Pseudogene-Derived Endogenous siRNAs and Their Function
  17. Altmetric Badge
    Chapter 16 Methods to Study Translated Pseudogenes: In Vitro Translation, Fusion with a Tag/Reporter Gene, and Complementation Assay
  18. Altmetric Badge
    Chapter 17 Targeted and robust amplification of mitochondrial DNA in the presence of nuclear-encoded mitochondrial pseudogenes using φ29 DNA polymerases.
  19. Altmetric Badge
    Chapter 18 Pseudogenes
  20. Altmetric Badge
    Chapter 19 Mutational Analysis of CYP21A2 Gene and CYP21A1P Pseudogene: Long-range PCR on Genomic DNA.
  21. Altmetric Badge
    Chapter 20 PMS2 Gene Mutational Analysis: Direct cDNA Sequencing to Circumvent Pseudogene Interference
  22. Altmetric Badge
    Chapter 21 Dealing with Pseudogenes in Molecular Diagnostics in the Next-Generation Sequencing Era
Attention for Chapter 19: Mutational Analysis of CYP21A2 Gene and CYP21A1P Pseudogene: Long-range PCR on Genomic DNA.
Altmetric Badge

Mentioned by

twitter
1 X user

Citations

dimensions_citation
5 Dimensions

Readers on

mendeley
19 Mendeley
Summary X Dimensions citations
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Mutational Analysis of CYP21A2 Gene and CYP21A1P Pseudogene: Long-range PCR on Genomic DNA.
Chapter number 19
Book title
Pseudogenes
Published in
Methods in molecular biology, April 2014
DOI 10.1007/978-1-4939-0835-6_19
Pubmed ID
24823785
Book ISBNs
978-1-4939-0834-9, 978-1-4939-0835-6
Authors

Hsien-Hsiung Lee, Hsien-Hsiung Lee B.S.

Editors

Laura Poliseno

Abstract

CYP21A2, the gene that codes for P450c21 (Steroid 21-hydroxylase), has a duplicated pseudogene called CYP21A1P. The gene and the pseudogene share 98 % and 96 % sequence homology in exons and in noncoding sequences, respectively, and are located 30 kb apart within the HLA class III human histocompatibility complex locus on chromosome 6p21.3. CYP21A1P is inactive due to the presence of 11 deteriorated mutations in its coding region. These mutations can be transferred to the functional CYP21A2 through intergenic recombination during meiosis or mitosis and lead to the congenital adrenal hyperplasia (CAH) resulting from 21-hydroxylase deficiency. Conversely, portions of CYP21A2 sequence can be transferred to CYP21A1P, modifying the haplotype. Here, we describe a well-established protocol that can be used to unambiguously study the mutational profile of CYP21A2 gene and CYP21A1P pseudogene. The protocol is based on long-range PCR amplification with allele-specific primers, followed by DNA sequencing of smaller fragments.

View on publisher site
X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 19 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 3 16%
Student > Master 3 16%
Researcher 3 16%
Other 2 11%
Student > Ph. D. Student 1 5%
Other 1 5%
Unknown 6 32%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 26%
Agricultural and Biological Sciences 2 11%
Medicine and Dentistry 2 11%
Immunology and Microbiology 1 5%
Chemistry 1 5%
Other 0 0%
Unknown 8 42%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 May 2014.
All research outputs
#20,229,658
of 22,755,127 outputs
Outputs from Methods in molecular biology
#9,863
of 13,089 outputs
Outputs of similar age
#193,267
of 227,088 outputs
Outputs of similar age from Methods in molecular biology
#95
of 145 outputs
Altmetric has tracked 22,755,127 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,089 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 227,088 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 145 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

This page is provided by Altmetric.