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Membrane Protein Structure and Function Characterization

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Cover of 'Membrane Protein Structure and Function Characterization'

Table of Contents

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    Book Overview
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    Chapter 1 Recombinant Overexpression of Mammalian TSPO Isoforms 1 and 2
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    Chapter 2 Functional Assembly of Soluble and Membrane Recombinant Proteins of Mammalian NADPH Oxidase Complex
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    Chapter 3 Direct Extraction and Purification of Recombinant Membrane Proteins from Pichia pastoris Protoplasts
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    Chapter 4 Cell-Free Expression for the Study of Hydrophobic Proteins: The Example of Yeast ATP-Synthase Subunits
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    Chapter 5 Wheat Germ Cell-Free Overexpression for the Production of Membrane Proteins
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    Chapter 6 Methyl-Specific Isotope Labeling Strategies for NMR Studies of Membrane Proteins
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    Chapter 7 Labeling of Membrane Complexes for Electron Microscopy
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    Chapter 8 Expression, Biochemistry, and Stabilization with Camel Antibodies of Membrane Proteins: Case Study of the Mouse 5-HT3 Receptor
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    Chapter 9 Characterization of New Detergents and Detergent Mimetics by Scattering Techniques for Membrane Protein Crystallization
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    Chapter 10 Secondary Structure Determination by Means of ATR-FTIR Spectroscopy
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    Chapter 11 Native Mass Spectrometry for the Characterization of Structure and Interactions of Membrane Proteins
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    Chapter 12 Mass Spectrometry of Mitochondrial Membrane Protein Complexes
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    Chapter 13 Functional Studies on Membrane Proteins by Means of H/D Exchange in Infrared: Structural Changes in Na+ NQR from V. cholerae in the Presence of Lipids
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    Chapter 14 Reconstitution of Membrane Proteins in Liposomes
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    Chapter 15 Ion Channels as Reporters of Membrane Receptor Function: Automated Analysis in Xenopus Oocytes
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    Chapter 16 The CRACAM Robot: Two-Dimensional Crystallization of Membrane Protein
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    Chapter 17 Reconstitution of Membrane Proteins into Nanodiscs for Single-Particle Electron Microscopy
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    Chapter 18 Solid-State NMR of Membrane Protein Reconstituted in Proteoliposomes, the Case of TSPO
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    Chapter 19 Sample Preparation for Membrane Protein Structural Studies by Solid-State NMR
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    Chapter 20 Simulation of Ligand Binding to Membrane Proteins
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    Chapter 21 Molecular Modeling of Transporters: From Low Resolution Cryo-Electron Microscopy Map to Conformational Exploration. The Example of TSPO
Attention for Chapter 10: Secondary Structure Determination by Means of ATR-FTIR Spectroscopy
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Chapter title
Secondary Structure Determination by Means of ATR-FTIR Spectroscopy
Chapter number 10
Book title
Membrane Protein Structure and Function Characterization
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7151-0_10
Pubmed ID
Book ISBNs
978-1-4939-7149-7, 978-1-4939-7151-0
Authors

Batoul Srour, Stefan Bruechert, Susana L. A. Andrade, Petra Hellwig, Srour, Batoul, Bruechert, Stefan, Andrade, Susana L. A., Hellwig, Petra

Abstract

Specialized infrared spectroscopic techniques have been developed that allow studying the secondary structure of membrane proteins and the influence of crucial parameters like lipid content and detergent. Here, we focus on an ATR-FTIR spectroscopic study of Af-Amt1 and the influence of LDAO/glycerol on its structural integrity. Our results clearly indicate that infrared spectroscopy can be used to identify the adapted sample conditions.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 39 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 39 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 21%
Student > Doctoral Student 4 10%
Researcher 4 10%
Student > Master 4 10%
Other 3 8%
Other 6 15%
Unknown 10 26%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 18%
Biochemistry, Genetics and Molecular Biology 5 13%
Chemistry 4 10%
Materials Science 3 8%
Pharmacology, Toxicology and Pharmaceutical Science 2 5%
Other 4 10%
Unknown 14 36%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 April 2018.
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#20,879,072
of 23,498,099 outputs
Outputs from Methods in molecular biology
#10,161
of 13,368 outputs
Outputs of similar age
#358,560
of 423,677 outputs
Outputs of similar age from Methods in molecular biology
#841
of 1,071 outputs
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So far Altmetric has tracked 13,368 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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