Chapter title |
Reconstitution of Membrane Proteins in Liposomes
|
---|---|
Chapter number | 14 |
Book title |
Membrane Protein Structure and Function Characterization
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7151-0_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7149-7, 978-1-4939-7151-0
|
Authors |
Alice Verchère, Isabelle Broutin, Martin Picard |
Abstract |
Membrane protein reconstitution in liposomes is an invaluable technique to study numerous properties of membrane proteins in vitro. Kinetics, substrate specificity, and protein-protein interaction of membrane proteins can be investigated once they are embedded in the liposome bilayer. Both protocols described here aim to investigate the efflux pump MexA-MexB-OprM from Pseudomonas aeruginosa, a proteins complex embedded in both membranes of this Gram-negative bacteria. This tri-partite system, which by-passes the periplams, is involved in antibiotic resistance. First, we describe a protocol to study MexB, the actual transporter, and second we propose an alternate protocol where the tripartite system is investigated as a whole. |
X Demographics
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Demographic breakdown
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Mendeley readers
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Researcher | 40 | 16% |
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Student > Doctoral Student | 9 | 4% |
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Physics and Astronomy | 6 | 2% |
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Unknown | 54 | 22% |