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Membrane Protein Structure and Function Characterization

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Cover of 'Membrane Protein Structure and Function Characterization'

Table of Contents

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    Book Overview
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    Chapter 1 Recombinant Overexpression of Mammalian TSPO Isoforms 1 and 2
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    Chapter 2 Functional Assembly of Soluble and Membrane Recombinant Proteins of Mammalian NADPH Oxidase Complex
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    Chapter 3 Direct Extraction and Purification of Recombinant Membrane Proteins from Pichia pastoris Protoplasts
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    Chapter 4 Cell-Free Expression for the Study of Hydrophobic Proteins: The Example of Yeast ATP-Synthase Subunits
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    Chapter 5 Wheat Germ Cell-Free Overexpression for the Production of Membrane Proteins
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    Chapter 6 Methyl-Specific Isotope Labeling Strategies for NMR Studies of Membrane Proteins
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    Chapter 7 Labeling of Membrane Complexes for Electron Microscopy
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    Chapter 8 Expression, Biochemistry, and Stabilization with Camel Antibodies of Membrane Proteins: Case Study of the Mouse 5-HT3 Receptor
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    Chapter 9 Characterization of New Detergents and Detergent Mimetics by Scattering Techniques for Membrane Protein Crystallization
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    Chapter 10 Secondary Structure Determination by Means of ATR-FTIR Spectroscopy
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    Chapter 11 Native Mass Spectrometry for the Characterization of Structure and Interactions of Membrane Proteins
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    Chapter 12 Mass Spectrometry of Mitochondrial Membrane Protein Complexes
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    Chapter 13 Functional Studies on Membrane Proteins by Means of H/D Exchange in Infrared: Structural Changes in Na+ NQR from V. cholerae in the Presence of Lipids
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    Chapter 14 Reconstitution of Membrane Proteins in Liposomes
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    Chapter 15 Ion Channels as Reporters of Membrane Receptor Function: Automated Analysis in Xenopus Oocytes
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    Chapter 16 The CRACAM Robot: Two-Dimensional Crystallization of Membrane Protein
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    Chapter 17 Reconstitution of Membrane Proteins into Nanodiscs for Single-Particle Electron Microscopy
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    Chapter 18 Solid-State NMR of Membrane Protein Reconstituted in Proteoliposomes, the Case of TSPO
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    Chapter 19 Sample Preparation for Membrane Protein Structural Studies by Solid-State NMR
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    Chapter 20 Simulation of Ligand Binding to Membrane Proteins
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    Chapter 21 Molecular Modeling of Transporters: From Low Resolution Cryo-Electron Microscopy Map to Conformational Exploration. The Example of TSPO
Attention for Chapter 14: Reconstitution of Membrane Proteins in Liposomes
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Chapter title
Reconstitution of Membrane Proteins in Liposomes
Chapter number 14
Book title
Membrane Protein Structure and Function Characterization
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7151-0_14
Pubmed ID
Book ISBNs
978-1-4939-7149-7, 978-1-4939-7151-0
Authors

Alice Verchère, Isabelle Broutin, Martin Picard

Abstract

Membrane protein reconstitution in liposomes is an invaluable technique to study numerous properties of membrane proteins in vitro. Kinetics, substrate specificity, and protein-protein interaction of membrane proteins can be investigated once they are embedded in the liposome bilayer. Both protocols described here aim to investigate the efflux pump MexA-MexB-OprM from Pseudomonas aeruginosa, a proteins complex embedded in both membranes of this Gram-negative bacteria. This tri-partite system, which by-passes the periplams, is involved in antibiotic resistance. First, we describe a protocol to study MexB, the actual transporter, and second we propose an alternate protocol where the tripartite system is investigated as a whole.

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X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 247 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 1 <1%
Czechia 1 <1%
Canada 1 <1%
United States 1 <1%
Poland 1 <1%
Unknown 242 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 61 25%
Researcher 40 16%
Student > Master 33 13%
Student > Bachelor 25 10%
Student > Doctoral Student 9 4%
Other 28 11%
Unknown 51 21%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 80 32%
Agricultural and Biological Sciences 46 19%
Chemistry 28 11%
Engineering 7 3%
Physics and Astronomy 6 2%
Other 26 11%
Unknown 54 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 July 2017.
All research outputs
#18,565,641
of 22,994,508 outputs
Outputs from Methods in molecular biology
#7,952
of 13,151 outputs
Outputs of similar age
#311,367
of 421,174 outputs
Outputs of similar age from Methods in molecular biology
#693
of 1,074 outputs
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So far Altmetric has tracked 13,151 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,074 others from the same source and published within six weeks on either side of this one. This one is in the 21st percentile – i.e., 21% of its contemporaries scored the same or lower than it.