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Membrane Protein Structure and Function Characterization

Overview of attention for book
Cover of 'Membrane Protein Structure and Function Characterization'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Recombinant Overexpression of Mammalian TSPO Isoforms 1 and 2
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    Chapter 2 Functional Assembly of Soluble and Membrane Recombinant Proteins of Mammalian NADPH Oxidase Complex
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    Chapter 3 Direct Extraction and Purification of Recombinant Membrane Proteins from Pichia pastoris Protoplasts
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    Chapter 4 Cell-Free Expression for the Study of Hydrophobic Proteins: The Example of Yeast ATP-Synthase Subunits
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    Chapter 5 Wheat Germ Cell-Free Overexpression for the Production of Membrane Proteins
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    Chapter 6 Methyl-Specific Isotope Labeling Strategies for NMR Studies of Membrane Proteins
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    Chapter 7 Labeling of Membrane Complexes for Electron Microscopy
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    Chapter 8 Expression, Biochemistry, and Stabilization with Camel Antibodies of Membrane Proteins: Case Study of the Mouse 5-HT3 Receptor
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    Chapter 9 Characterization of New Detergents and Detergent Mimetics by Scattering Techniques for Membrane Protein Crystallization
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    Chapter 10 Secondary Structure Determination by Means of ATR-FTIR Spectroscopy
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    Chapter 11 Native Mass Spectrometry for the Characterization of Structure and Interactions of Membrane Proteins
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    Chapter 12 Mass Spectrometry of Mitochondrial Membrane Protein Complexes
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    Chapter 13 Functional Studies on Membrane Proteins by Means of H/D Exchange in Infrared: Structural Changes in Na+ NQR from V. cholerae in the Presence of Lipids
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    Chapter 14 Reconstitution of Membrane Proteins in Liposomes
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    Chapter 15 Ion Channels as Reporters of Membrane Receptor Function: Automated Analysis in Xenopus Oocytes
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    Chapter 16 The CRACAM Robot: Two-Dimensional Crystallization of Membrane Protein
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    Chapter 17 Reconstitution of Membrane Proteins into Nanodiscs for Single-Particle Electron Microscopy
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    Chapter 18 Solid-State NMR of Membrane Protein Reconstituted in Proteoliposomes, the Case of TSPO
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    Chapter 19 Sample Preparation for Membrane Protein Structural Studies by Solid-State NMR
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    Chapter 20 Simulation of Ligand Binding to Membrane Proteins
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    Chapter 21 Molecular Modeling of Transporters: From Low Resolution Cryo-Electron Microscopy Map to Conformational Exploration. The Example of TSPO
Attention for Chapter 7: Labeling of Membrane Complexes for Electron Microscopy
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (53rd percentile)
  • Good Attention Score compared to outputs of the same age and source (72nd percentile)

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Chapter title
Labeling of Membrane Complexes for Electron Microscopy
Chapter number 7
Book title
Membrane Protein Structure and Function Characterization
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7151-0_7
Pubmed ID
Book ISBNs
978-1-4939-7149-7, 978-1-4939-7151-0
Authors

Francesca Gubellini, Rémi Fronzes, Gubellini, Francesca, Fronzes, Rémi

Abstract

Localization of specific subunits or domains of interest inside protein complexes can be challenging, especially for membrane machineries. The amphipatic nature of their subunits and their modular organization results in difficult genetic manipulation and instability upon purification. Here, we present different labeling approaches that have been demonstrated successful in the structural characterization of large membrane complexes.

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X Demographics

The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 67%
Unknown 1 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 67%
Unknown 1 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 August 2017.
All research outputs
#12,757,102
of 22,996,001 outputs
Outputs from Methods in molecular biology
#3,172
of 13,151 outputs
Outputs of similar age
#195,205
of 421,191 outputs
Outputs of similar age from Methods in molecular biology
#292
of 1,074 outputs
Altmetric has tracked 22,996,001 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,151 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done well, scoring higher than 75% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 421,191 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.
We're also able to compare this research output to 1,074 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 72% of its contemporaries.