| Chapter title |
Solid-State NMR of Membrane Protein Reconstituted in Proteoliposomes, the Case of TSPO
|
|---|---|
| Chapter number | 18 |
| Book title |
Membrane Protein Structure and Function Characterization
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7151-0_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7149-7, 978-1-4939-7151-0
|
| Authors |
Lucile Senicourt, Luminita Duma, Vassilios Papadopoulos, Jean-Jacques Lacapere |
| Abstract |
Structural studies of membrane proteins (MP) in a native or native-like environment remain a challenge. X-ray crystallography of three-dimensional crystals of MP in lipids and cryo-electron microscopy of two-dimensional crystals also in lipids have given atomic structures of several MP. Recent developments of solid-state NMR (ssNMR) provided structural data of MP in lipids and should give access to the dynamic behavior of MP's in a native-like environment. Preparation of samples for ssNMR is not trivial with overexpressed proteins since purified recombinant MP have to be reincorporated in proteoliposomes and concentrated in the small volume of the rotor used for ssNMR studies. We present here the protocol that we have used to study the recombinant mouse TSPO1, an integral membrane protein of 20 kDa mostly found in the outer membrane of mitochondria and overexpressed in E. coli bacteria. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 27% |
| Researcher | 2 | 18% |
| Professor | 1 | 9% |
| Student > Bachelor | 1 | 9% |
| Unspecified | 1 | 9% |
| Other | 0 | 0% |
| Unknown | 3 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 27% |
| Chemistry | 2 | 18% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 9% |
| Psychology | 1 | 9% |
| Unspecified | 1 | 9% |
| Other | 0 | 0% |
| Unknown | 3 | 27% |