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Membrane Protein Structure and Function Characterization

Overview of attention for book
Cover of 'Membrane Protein Structure and Function Characterization'

Table of Contents

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    Book Overview
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    Chapter 1 Recombinant Overexpression of Mammalian TSPO Isoforms 1 and 2
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    Chapter 2 Functional Assembly of Soluble and Membrane Recombinant Proteins of Mammalian NADPH Oxidase Complex
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    Chapter 3 Direct Extraction and Purification of Recombinant Membrane Proteins from Pichia pastoris Protoplasts
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    Chapter 4 Cell-Free Expression for the Study of Hydrophobic Proteins: The Example of Yeast ATP-Synthase Subunits
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    Chapter 5 Wheat Germ Cell-Free Overexpression for the Production of Membrane Proteins
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    Chapter 6 Methyl-Specific Isotope Labeling Strategies for NMR Studies of Membrane Proteins
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    Chapter 7 Labeling of Membrane Complexes for Electron Microscopy
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    Chapter 8 Expression, Biochemistry, and Stabilization with Camel Antibodies of Membrane Proteins: Case Study of the Mouse 5-HT3 Receptor
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    Chapter 9 Characterization of New Detergents and Detergent Mimetics by Scattering Techniques for Membrane Protein Crystallization
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    Chapter 10 Secondary Structure Determination by Means of ATR-FTIR Spectroscopy
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    Chapter 11 Native Mass Spectrometry for the Characterization of Structure and Interactions of Membrane Proteins
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    Chapter 12 Mass Spectrometry of Mitochondrial Membrane Protein Complexes
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    Chapter 13 Functional Studies on Membrane Proteins by Means of H/D Exchange in Infrared: Structural Changes in Na+ NQR from V. cholerae in the Presence of Lipids
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    Chapter 14 Reconstitution of Membrane Proteins in Liposomes
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    Chapter 15 Ion Channels as Reporters of Membrane Receptor Function: Automated Analysis in Xenopus Oocytes
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    Chapter 16 The CRACAM Robot: Two-Dimensional Crystallization of Membrane Protein
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    Chapter 17 Reconstitution of Membrane Proteins into Nanodiscs for Single-Particle Electron Microscopy
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    Chapter 18 Solid-State NMR of Membrane Protein Reconstituted in Proteoliposomes, the Case of TSPO
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    Chapter 19 Sample Preparation for Membrane Protein Structural Studies by Solid-State NMR
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    Chapter 20 Simulation of Ligand Binding to Membrane Proteins
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    Chapter 21 Molecular Modeling of Transporters: From Low Resolution Cryo-Electron Microscopy Map to Conformational Exploration. The Example of TSPO
Attention for Chapter 21: Molecular Modeling of Transporters: From Low Resolution Cryo-Electron Microscopy Map to Conformational Exploration. The Example of TSPO
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (51st percentile)
  • Good Attention Score compared to outputs of the same age and source (70th percentile)

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Chapter title
Molecular Modeling of Transporters: From Low Resolution Cryo-Electron Microscopy Map to Conformational Exploration. The Example of TSPO
Chapter number 21
Book title
Membrane Protein Structure and Function Characterization
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7151-0_21
Pubmed ID
Book ISBNs
978-1-4939-7149-7, 978-1-4939-7151-0
Authors

Aurore Vaitinadapoule, Catherine Etchebest

Abstract

This chapter describes a protocol to establish a three-dimensional (3D) model of a protein and to explore its conformational landscape. It combines predictions from up-to-date bioinformatics methods with low-resolution experimental data. It also proposes to examine rapidly the dynamics of the protein using molecular dynamics simulations with a coarse-grained force field. Tools for analyzing these trajectories are suggested as well as those for constructing all-atoms models. Thus, starting from a protein sequence and using free software, the user can get important conformational information, which might improve the knowledge about the protein function.

X Demographics

X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 40%
Unspecified 1 20%
Researcher 1 20%
Unknown 1 20%
Readers by discipline Count As %
Unspecified 1 20%
Computer Science 1 20%
Physics and Astronomy 1 20%
Chemistry 1 20%
Unknown 1 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 November 2017.
All research outputs
#13,050,701
of 22,994,508 outputs
Outputs from Methods in molecular biology
#3,314
of 13,151 outputs
Outputs of similar age
#201,375
of 421,174 outputs
Outputs of similar age from Methods in molecular biology
#307
of 1,074 outputs
Altmetric has tracked 22,994,508 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,151 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 73% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 421,174 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.
We're also able to compare this research output to 1,074 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.