| Chapter title |
Assessment of pathological and physiological changes in mouse lung through bronchoalveolar lavage.
|
|---|---|
| Chapter number | 3 |
| Book title |
Molecular Toxicology Protocols
|
| Published in |
Methods in molecular biology, March 2014
|
| DOI | 10.1007/978-1-62703-739-6_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-62703-738-9, 978-1-62703-739-6
|
| Authors |
Di YP, Yuanpu Peter Di |
| Editors |
Phouthone Keohavong, Stephen G. Grant |
| Abstract |
In animals, environmental exposure such as toxic chemicals and microorganisms or pathophysiological conditions in respiratory system could result in inflammatory response in their lungs. Bronchoalveolar lavage (BAL) is a procedure that can be used to collect samples from animal lungs to efficiently evaluate the immune response by examining both the compositions of cells and fluid from lavage. The profile of inflammatory cells in BAL provides a qualitative description of inflammatory response and the secretion in BAL fluid contains proteins of inflammatory mediators and albumin as a quantitative measurement of inflammation and tissue injury in the lungs. A consistent experimental approach on how to lavage mouse lungs and collect samples is important for a reproducible evaluation of pathological and physiological changes in mouse lung especially for the analysis of inflammation. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Librarian | 1 | 25% |
| Student > Ph. D. Student | 1 | 25% |
| Researcher | 1 | 25% |
| Student > Doctoral Student | 1 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 2 | 50% |
| Chemistry | 1 | 25% |
| Medicine and Dentistry | 1 | 25% |