Chapter title |
Proteomic Analysis of the Asthmatic Airway
|
---|---|
Chapter number | 14 |
Book title |
Heterogeneity in Asthma
|
Published in |
Advances in experimental medicine and biology, January 2014
|
DOI | 10.1007/978-1-4614-8603-9_14 |
Pubmed ID | |
Book ISBNs |
978-1-4614-8602-2, 978-1-4614-8603-9
|
Authors |
John E. Wiktorowicz, Mohammad Jamaluddin, Wiktorowicz JE, Jamaluddin M, Wiktorowicz, John E., Jamaluddin, Mohammad |
Abstract |
Proteomic investigations in general utilize varied technologies for sample preparation, separations, quantification, protein identification, and biological rationalization. Their applications range from pure discovery and mechanistic studies to biomarker discovery/verification/validation. In each specific case, the analytical strategy to be implemented is tailored to the type of sample that serves as the target of the investigations. Proteomic investigations take into consideration sample complexity, the cellular heterogeneity (particularly from tissues), the potential dynamic range of the protein and peptide abundance within the sample, the likelihood of posttranslational modifications (PTM), and other important factors that might influence the final output of the study. We describe the sample types typically used for proteomic investigations into the biology of asthma and review the most recent related publications with special attention to those that deal with the unique airway samples such as bronchoalveolar lavage fluids (BALF), epithelial lining fluid and cells (ELF), induced sputum (IS), and exhaled breath condensate (EBC). Finally, we describe the newest proteomics approaches to sample preparation of the unique airway samples, BALF and IS. |
X Demographics
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Unknown | 1 | 50% |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
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Professor > Associate Professor | 4 | 19% |
Researcher | 4 | 19% |
Student > Ph. D. Student | 3 | 14% |
Other | 1 | 5% |
Student > Doctoral Student | 1 | 5% |
Other | 3 | 14% |
Unknown | 5 | 24% |
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Biochemistry, Genetics and Molecular Biology | 1 | 5% |
Unspecified | 1 | 5% |
Nursing and Health Professions | 1 | 5% |
Other | 0 | 0% |
Unknown | 6 | 29% |