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Mendeley readers
Chapter title |
Use of Minigenome Systems to Study RSV Transcription
|
---|---|
Chapter number | 11 |
Book title |
Human Respiratory Syncytial Virus
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3687-8_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3685-4, 978-1-4939-3687-8
|
Authors |
Michael N. Teng, Kim C. Tran |
Abstract |
Minigenome assays have been essential tools in the understanding of viral transcription and RNA replication for respiratory syncytial virus (RSV). Here, we describe the RSV minigenome assay for determining transcription by the viral polymerase in the absence of infection. We detail two different methods of detecting viral RNA synthesis: a firefly luciferase assay for rapid and sensitive measurement of RSV polymerase activity; and a real-time quantitative PCR method for determination of specific effects on the transcription of individual viral genes and the polar transcription gradient of RSV. |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 23% |
Student > Master | 3 | 23% |
Student > Bachelor | 2 | 15% |
Researcher | 1 | 8% |
Unknown | 4 | 31% |
Readers by discipline | Count | As % |
---|---|---|
Immunology and Microbiology | 4 | 31% |
Biochemistry, Genetics and Molecular Biology | 2 | 15% |
Medicine and Dentistry | 1 | 8% |
Engineering | 1 | 8% |
Unknown | 5 | 38% |