Chapter title |
Respiratory Syncytial Virus (RSV): Neutralizing Antibody, a Correlate of Immune Protection
|
---|---|
Chapter number | 7 |
Book title |
Human Respiratory Syncytial Virus
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3687-8_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3685-4, 978-1-4939-3687-8
|
Authors |
Pedro A. Piedra, Anne M. Hause, Letisha Aideyan |
Abstract |
Assays that measure RSV-specific neutralizing antibody activity are very useful for evaluating vaccine candidates, performing seroprevalence studies, and detecting infection. Neutralizing antibody activity is normally measured by a plaque reduction neutralization assay or by a microneutralization assay with or without complement. These assays measure the functional capacity of serum (or other fluids) to neutralize virus infectivity in cells as compared to ELISA assays that only measure the binding capacity against an antigen. This chapter discusses important elements in standardization of the RSV-specific microneutralization assay for use in the laboratory. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 30 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 6 | 20% |
Researcher | 5 | 17% |
Student > Ph. D. Student | 5 | 17% |
Professor > Associate Professor | 2 | 7% |
Lecturer > Senior Lecturer | 1 | 3% |
Other | 2 | 7% |
Unknown | 9 | 30% |
Readers by discipline | Count | As % |
---|---|---|
Immunology and Microbiology | 9 | 30% |
Social Sciences | 2 | 7% |
Agricultural and Biological Sciences | 2 | 7% |
Business, Management and Accounting | 1 | 3% |
Physics and Astronomy | 1 | 3% |
Other | 1 | 3% |
Unknown | 14 | 47% |