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Human Respiratory Syncytial Virus

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Cover of 'Human Respiratory Syncytial Virus'

Table of Contents

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    Book Overview
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    Chapter 1 Human Respiratory Syncytial Virus: An Introduction
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    Chapter 2 Human Respiratory Syncytial Virus
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    Chapter 3 Quantification of RSV Infectious Particles by Plaque Assay and Immunostaining Assay
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    Chapter 4 Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays
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    Chapter 5 Secretory Expression and Purification of Respiratory Syncytial Virus G and F Proteins in Human Cells
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    Chapter 6 Development of Human Monoclonal Antibodies Against Respiratory Syncytial Virus Using a High Efficiency Human Hybridoma Technique
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    Chapter 7 Respiratory Syncytial Virus (RSV): Neutralizing Antibody, a Correlate of Immune Protection
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    Chapter 8 Host Factors Modulating RSV Infection: Use of Small Interfering RNAs to Probe Functional Importance
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    Chapter 9 In Vitro Modeling of RSV Infection and Cytopathogenesis in Well-Differentiated Human Primary Airway Epithelial Cells (WD-PAECs)
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    Chapter 10 Reverse Genetics of Respiratory Syncytial Virus
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    Chapter 11 Use of Minigenome Systems to Study RSV Transcription
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    Chapter 12 Screening for Host Factors Directly Interacting with RSV Protein: Microfluidics
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    Chapter 13 A Proteomic-Based Workflow Using Purified Respiratory Syncytial Virus Particles to Identify Cellular Factors as Drug Targets
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    Chapter 14 Human Respiratory Syncytial Virus
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    Chapter 15 Mouse and Cotton Rat Models of Human Respiratory Syncytial Virus
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    Chapter 16 Human Respiratory Syncytial Virus
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    Chapter 17 Evaluation of the Adaptive Immune Response to Respiratory Syncytial Virus
Attention for Chapter 7: Respiratory Syncytial Virus (RSV): Neutralizing Antibody, a Correlate of Immune Protection
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Chapter title
Respiratory Syncytial Virus (RSV): Neutralizing Antibody, a Correlate of Immune Protection
Chapter number 7
Book title
Human Respiratory Syncytial Virus
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3687-8_7
Pubmed ID
Book ISBNs
978-1-4939-3685-4, 978-1-4939-3687-8
Authors

Pedro A. Piedra, Anne M. Hause, Letisha Aideyan

Abstract

Assays that measure RSV-specific neutralizing antibody activity are very useful for evaluating vaccine candidates, performing seroprevalence studies, and detecting infection. Neutralizing antibody activity is normally measured by a plaque reduction neutralization assay or by a microneutralization assay with or without complement. These assays measure the functional capacity of serum (or other fluids) to neutralize virus infectivity in cells as compared to ELISA assays that only measure the binding capacity against an antigen. This chapter discusses important elements in standardization of the RSV-specific microneutralization assay for use in the laboratory.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 30 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 6 20%
Researcher 5 17%
Student > Ph. D. Student 5 17%
Professor > Associate Professor 2 7%
Lecturer > Senior Lecturer 1 3%
Other 2 7%
Unknown 9 30%
Readers by discipline Count As %
Immunology and Microbiology 9 30%
Social Sciences 2 7%
Agricultural and Biological Sciences 2 7%
Business, Management and Accounting 1 3%
Physics and Astronomy 1 3%
Other 1 3%
Unknown 14 47%