Chapter title |
Development of Human Monoclonal Antibodies Against Respiratory Syncytial Virus Using a High Efficiency Human Hybridoma Technique
|
---|---|
Chapter number | 6 |
Book title |
Human Respiratory Syncytial Virus
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3687-8_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3685-4, 978-1-4939-3687-8
|
Authors |
Gabriela Alvarado, James E. CroweJr., James E. Crowe |
Abstract |
Human monoclonal antibodies against RSV have high potential for use as prophylaxis or therapeutic molecules, and they also can be used to define the structure of protective epitopes for rational vaccine design. In the past, however, isolation of human monoclonal antibodies was difficult and inefficient. Here, we describe contemporary methods for activation and proliferation of primary human memory B cells followed by cytofusion to non-secreting myeloma cells by dielectrophoresis to generate human hybridomas secreting RSV-specific monoclonal antibodies. We also provide experimental methods for screening human B cell lines to obtain RSV-specific lines, especially lines secreting neutralizing antibodies. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Canada | 1 | 8% |
Unknown | 11 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 17% |
Student > Master | 2 | 17% |
Lecturer | 1 | 8% |
Other | 1 | 8% |
Professor | 1 | 8% |
Other | 0 | 0% |
Unknown | 5 | 42% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 2 | 17% |
Immunology and Microbiology | 2 | 17% |
Medicine and Dentistry | 1 | 8% |
Engineering | 1 | 8% |
Unknown | 6 | 50% |