Chapter title |
Comprehensive Mitochondrial Genome Analysis by Massively Parallel Sequencing.
|
---|---|
Chapter number | 1 |
Book title |
Mitochondrial DNA
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3040-1_1 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3039-5, 978-1-4939-3040-1
|
Authors |
Palculict, Meagan E, Zhang, Victor Wei, Wong, Lee-Jun, Wang, Jing, Meagan E. Palculict, Victor Wei Zhang, Lee-Jun Wong, Jing Wang |
Abstract |
Next-generation sequencing (NGS) based on massively parallel sequencing (MPS) of the entire 16,569 bp mitochondrial genome generates thousands of reads for each nucleotide position. The high-throughput sequence data generated allow the detection of mitochondrial DNA (mtDNA) point mutations and deletions with the ability to accurately quantify the mtDNA point mutation heteroplasmy and to determine the deletion breakpoints. In addition, this method is particularly sensitive for the detection of low-level mtDNA large deletions and multiple deletions. It is by far the most powerful tool for molecular diagnosis of mtDNA disorders. |
X Demographics
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 23 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Professor > Associate Professor | 5 | 22% |
Researcher | 4 | 17% |
Student > Bachelor | 3 | 13% |
Student > Doctoral Student | 2 | 9% |
Other | 1 | 4% |
Other | 3 | 13% |
Unknown | 5 | 22% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 9 | 39% |
Agricultural and Biological Sciences | 4 | 17% |
Neuroscience | 2 | 9% |
Veterinary Science and Veterinary Medicine | 1 | 4% |
Medicine and Dentistry | 1 | 4% |
Other | 1 | 4% |
Unknown | 5 | 22% |