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Mendeley readers
Chapter title |
Import of Fluorescent RNA into Mitochondria of Living Cells
|
---|---|
Chapter number | 13 |
Book title |
Mitochondrial DNA
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Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3040-1_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3039-5, 978-1-4939-3040-1
|
Authors |
Jaroslav Zelenka, Petr Ježek |
Abstract |
Methods of in vivo visualization and manipulation of mitochondrial genetic machinery are limited due to the need to surpass not only the cytoplasmic membrane but also two mitochondrial membranes. Here, we employ the matrix-addressing sequence of mitochondrial ribosomal 5S-rRNA (termed MAM), which is naturally imported into mammalian mitochondria, to construct an import system for in vivo targeting of mitochondrial (mt) DNA or mtRNA, in order to provide fluorescence hybridization of the desired sequences. |
Mendeley readers
The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 56% |
Student > Bachelor | 1 | 11% |
Student > Master | 1 | 11% |
Unknown | 2 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 44% |
Agricultural and Biological Sciences | 2 | 22% |
Unknown | 3 | 33% |