| Chapter title |
Implanting Glioblastoma Spheroids into Rat Brains and Monitoring Tumor Growth by MRI Volumetry
|
|---|---|
| Chapter number | 12 |
| Book title |
RNAi and Small Regulatory RNAs in Stem Cells
|
| Published in |
Methods in molecular biology, July 2017
|
| DOI | 10.1007/978-1-4939-7108-4_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7106-0, 978-1-4939-7108-4
|
| Authors |
Mario Löhr, Thomas Linsenmann, Anna Jawork, Almuth F. Kessler, Nils Timmermann, György A. Homola, Ralf-Ingo Ernestus, Carsten Hagemann |
| Editors |
Baohong Zhang |
| Abstract |
The outcome of patients suffering from glioblastoma multiforme (GBM) remains poor with a median survival of less than 15 months. To establish innovative therapeutical approaches or to analyze the effect of protein overexpression or protein knockdown by RNA interference in vivo, animal models are mandatory. Here, we describe the implantation of C6 glioma spheroids into the rats' brain and how to follow tumor growth by MRI scans. We show that C6 cells grown in Sprague-Dawley rats share several morphologic features of human glioblastoma like pleomorphic cells, areas of necrosis, vascular proliferation, and tumor cell invasion into the surrounding brain tissue. In addition, we describe a method for tumor volumetry utilizing the CISS 3D- or contrast-enhanced T1-weighted 3D sequence and freely available post-processing software. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 43% |
| Unspecified | 1 | 14% |
| Professor > Associate Professor | 1 | 14% |
| Student > Bachelor | 1 | 14% |
| Researcher | 1 | 14% |
| Other | 0 | 0% |
| Readers by discipline | Count | As % |
|---|---|---|
| Unspecified | 1 | 14% |
| Biochemistry, Genetics and Molecular Biology | 1 | 14% |
| Mathematics | 1 | 14% |
| Agricultural and Biological Sciences | 1 | 14% |
| Physics and Astronomy | 1 | 14% |
| Other | 2 | 29% |