Chapter title |
RNAi Knockdown of Ape1 Gene in the Differentiation of Mouse Embryonic Stem Cells
|
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Chapter number | 10 |
Book title |
RNAi and Small Regulatory RNAs in Stem Cells
|
Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-7108-4_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7106-0, 978-1-4939-7108-4
|
Authors |
Gang-Ming Zou M.D., Ph.D., Jieqing Yu, Cynthia LeBron, Yumei Fu, Gang-Ming Zou |
Editors |
Baohong Zhang |
Abstract |
Murine embryonic stem cells (ES) are pluripotent cells and have the potential to become a wide variety of specialized cell types. Mouse ES cell differentiation can be regarded as a valuable biological tool that has led to major advances in our understanding of cell and developmental biology. In vitro differentiation of mouse ES cells can be directed to a specific lineage formation, such as hematopoietic lineage, by appropriate cytokine and/or growth factor stimulation. To study specific gene function in early developmental events, gene knockout approaches have been traditionally used, however, this is a time-consuming and expensive approach. Recently, we have shown that siRNA is an effective strategy to knock down target gene expression, such as Ape1, during ES cell differentiation, and consequently, one can alter cell fates in ES-derived differentiated cells. This approach will be applicable to test the function of a wide variety of gene products using the ES cell differentiation system. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 1 | 50% |
Student > Doctoral Student | 1 | 50% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 1 | 50% |
Unknown | 1 | 50% |