| Chapter title |
Forward RNAi Screens in Human Hematopoietic Stem Cells
|
|---|---|
| Chapter number | 3 |
| Book title |
RNAi and Small Regulatory RNAs in Stem Cells
|
| Published in |
Methods in molecular biology, July 2017
|
| DOI | 10.1007/978-1-4939-7108-4_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7106-0, 978-1-4939-7108-4
|
| Authors |
Galeev, Roman, Karlsson, Christine, Baudet, Aurélie, Larsson, Jonas, Roman Galeev, Christine Karlsson, Aurélie Baudet, Jonas Larsson |
| Editors |
Baohong Zhang |
| Abstract |
Identifying the genes and pathways that regulate self-renewal and differentiation in somatic stem cells is a central goal in stem cell and cancer biology. Here, we describe a method for RNA interference (RNAi)-based screens combined with next-generation sequencing (NGS) in primary human hematopoietic stem and progenitor cells (HSPCs). These cells are suitable targets for complex, selection-based screens using pooled lentiviral short hairpin RNA (shRNA) libraries. The screening approach presented in this chapter is a promising tool to dissect regulatory mechanisms in hematopoietic stem cells (HSCs) and somatic stem cells in general, and may be particularly useful to identify gene targets and modifiers that can be further exploited in strategies for ex vivo stem cell expansion. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Science communicators (journalists, bloggers, editors) | 1 | 50% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 1 | 20% |
| Student > Ph. D. Student | 1 | 20% |
| Student > Master | 1 | 20% |
| Researcher | 1 | 20% |
| Professor > Associate Professor | 1 | 20% |
| Other | 0 | 0% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 2 | 40% |
| Biochemistry, Genetics and Molecular Biology | 1 | 20% |
| Immunology and Microbiology | 1 | 20% |
| Nursing and Health Professions | 1 | 20% |