| Chapter title |
Liquid Culture Production of Fungal Microsclerotia
|
|---|---|
| Chapter number | 7 |
| Book title |
Microbial-Based Biopesticides
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-6367-6_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6365-2, 978-1-4939-6367-6
|
| Authors |
Mark A. Jackson, Angela R. Payne |
| Editors |
Travis R. Glare, Maria E. Moran-Diez |
| Abstract |
Fungal microsclerotia ("small" sclerotia) are compact hyphal aggregates, typically 50-600 μm in diameter, that are formed under unfavorable nutritional and/or environmental conditions. These structures are often melanized and desiccated to some degree containing endogenous nutritional reserves for use when favorable conditions return. Many fungi, mostly plant pathogens, produce microsclerotia as a survival structure. Liquid culture methods have been developed for producing microsclerotia of the Ascomycota Metarhizium spp, Colletotrichum truncatum, Mycoleptodiscus terrestris, and Trichoderma spp. While these fungi have varying culture conditions that optimize microsclerotia production, all share common nutritional and environmental requirements for microsclerotia formation. Described are the general liquid culture techniques, media components, and harvesting and drying methods necessary to produce stable microsclerotial granules of these fungi. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 20 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 4 | 20% |
| Student > Ph. D. Student | 3 | 15% |
| Researcher | 3 | 15% |
| Student > Bachelor | 2 | 10% |
| Unknown | 8 | 40% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 6 | 30% |
| Biochemistry, Genetics and Molecular Biology | 4 | 20% |
| Arts and Humanities | 1 | 5% |
| Medicine and Dentistry | 1 | 5% |
| Unknown | 8 | 40% |