You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
| Chapter title |
Purification of the Yersinia entomophaga Yen-TC Toxin Complex Using Size Exclusion Chromatography
|
|---|---|
| Chapter number | 4 |
| Book title |
Microbial-Based Biopesticides
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-6367-6_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6365-2, 978-1-4939-6367-6
|
| Authors |
Sandra A. Jones, Mark R. H. Hurst |
| Editors |
Travis R. Glare, Maria E. Moran-Diez |
| Abstract |
The Yersinia entomophaga toxin complex (Yen-TC) is the bacterium's main virulence determinant. Because of its high insect activity, methods were developed to allow the routine isolation and purification of Yen-TC from an overnight bacterial culture using size exclusion chromatography. Here we outline an overnight purification procedure using a 100-ml culture volume, where approximately 2 mg of Yen-TC, with an approximate purity of 95-98 %, can be routinely obtained. |
Mendeley readers
The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 100% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 100% |