Chapter title |
Production of Conidia by the Fungus Metarhizium anisopliae Using Solid-State Fermentation
|
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Chapter number | 6 |
Book title |
Microbial-Based Biopesticides
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-6367-6_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6365-2, 978-1-4939-6367-6
|
Authors |
Octavio Loera-Corral, Javier Porcayo-Loza, Roberto Montesinos-Matias, Ernesto Favela-Torres |
Editors |
Travis R. Glare, Maria E. Moran-Diez |
Abstract |
This chapter describes the production of conidia by Metarhizium anisopliae using solid-state fermentation. Before production of conidia, procedures for strains conservation, reactivation, and propagation are essential in order to provide genetic stability of the strains. The strain is conserved in freeze-dried vials and then reactivated through insect inoculation. Rice is used as a substrate for the conidia production in two different bioreactors: plastic bags and tubular bioreactor. The CO2 production in the tubular bioreactors is measured with a respirometer; this system allows calculating indirect growth parameters as lag time (tlag) (25-35 h), maximum rate of CO2 production (rCO2 max) (0.5-0.7 mg/gdm h), specific rate of CO2 production (μ) (0.10-0.15 1/h), and final CO2 production (CO2) (100-120 mg/gdm). Conidial yield per gram of dry substrate (gdm) should be above 1 × 10(9) conidia/gdm after 10 days of incubation. Germination and viability of conidia obtained after 10 days of incubation should be above 80 % and 75 %, respectively. Bioassays using of Tenebrio molitor as a host insect should yield a final mortality above 80 %. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 33 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 7 | 21% |
Professor | 3 | 9% |
Researcher | 3 | 9% |
Student > Ph. D. Student | 3 | 9% |
Professor > Associate Professor | 2 | 6% |
Other | 3 | 9% |
Unknown | 12 | 36% |
Readers by discipline | Count | As % |
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Environmental Science | 2 | 6% |
Immunology and Microbiology | 2 | 6% |
Veterinary Science and Veterinary Medicine | 1 | 3% |
Other | 0 | 0% |
Unknown | 13 | 39% |