| Chapter title |
Isolation of Exosomes from HTLV-Infected Cells
|
|---|---|
| Chapter number | 5 |
| Book title |
Human T-Lymphotropic Viruses
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6872-5_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6870-1, 978-1-4939-6872-5
|
| Authors |
Robert A. Barclay, Michelle L. Pleet, Yao Akpamagbo, Kinza Noor, Allison Mathiesen, Fatah Kashanchi, Barclay, Robert A., Pleet, Michelle L., Akpamagbo, Yao, Noor, Kinza, Mathiesen, Allison, Kashanchi, Fatah |
| Abstract |
Exosomes are small vesicles, approximately 30-100 nm in diameter, that transport various cargos, such as proteins and nucleic acids, between cells. It has been previously shown that exosomes can also transport viral proteins, such as the HTLV protein Tax, and viral RNAs, potentially contributing to disease pathogenesis. Therefore, it is important to understand their impact on recipient cells. Here, we describe methods of isolating and purifying exosomes from cell culture or tissue through ultracentrifugation, characterizing exosomes by surface biomarkers, and assays that evaluate the effect of exosomes on cells. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 21 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 24% |
| Researcher | 3 | 14% |
| Student > Master | 3 | 14% |
| Student > Bachelor | 1 | 5% |
| Professor | 1 | 5% |
| Other | 4 | 19% |
| Unknown | 4 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 29% |
| Agricultural and Biological Sciences | 4 | 19% |
| Medicine and Dentistry | 2 | 10% |
| Immunology and Microbiology | 2 | 10% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 5% |
| Other | 2 | 10% |
| Unknown | 4 | 19% |