Chapter title |
Methods for Identifying and Examining HTLV-1 HBZ Post-translational Modifications
|
---|---|
Chapter number | 9 |
Book title |
Human T-Lymphotropic Viruses
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6872-5_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6870-1, 978-1-4939-6872-5
|
Authors |
Jacob Al-Saleem, Mamuka Kvaratskhelia, Patrick L. Green, Al-Saleem, Jacob, Kvaratskhelia, Mamuka, Green, Patrick L. |
Abstract |
Post-translational modifications (PTMs) are chemical alterations to individual amino acids that alter a protein's conformation, stability, and/or function. Several pathogenic viruses have been shown to encode proteins with PTMs, including human T-cell leukemia virus type 1 (HTLV-1) Tax and Rex regulatory proteins. HTLV-1 basic leucine zipper protein (HBZ) was hypothesized to feature PTMs due to its functional activities and interactions with cellular transcription factors and acetyltransferases. Here, we describe the approach used to identify, via mass spectrometry, the PTMs of HBZ. In addition, we describe methods to determine the functional relevance of the identified PTMs. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 3 | 75% |
Researcher | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 2 | 50% |
Immunology and Microbiology | 1 | 25% |
Unknown | 1 | 25% |