| Chapter title |
Genome Editing in the Cricket, Gryllus bimaculatus
|
|---|---|
| Chapter number | 18 |
| Book title |
Genome Editing in Animals
|
| Published in |
Methods in molecular biology, June 2017
|
| DOI | 10.1007/978-1-4939-7128-2_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7127-5, 978-1-4939-7128-2
|
| Authors |
Takahito Watanabe, Sumihare Noji, Taro Mito, Watanabe, Takahito, Noji, Sumihare, Mito, Taro |
| Editors |
Izuho Hatada |
| Abstract |
Hemimetabolous, or incompletely metamorphosing, insects are phylogenetically basal and include many beneficial and deleterious species. The cricket, Gryllus bimaculatus, is an emerging model for hemimetabolous insects, based on the success of RNA interference (RNAi)-based gene-functional analyses and transgenic technology. Taking advantage of genome editing technologies in this species would greatly promote functional genomics studies. Genome editing has proven to be an effective method for site-specific genome manipulation in various species. Here, we describe a protocol for genome editing including gene knockout and gene knockin in G. bimaculatus for functional genomics studies. |
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Demographic breakdown
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Mendeley readers
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|---|---|---|
| Unknown | 17 | 100% |
Demographic breakdown
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| Professor > Associate Professor | 2 | 12% |
| Student > Ph. D. Student | 2 | 12% |
| Researcher | 2 | 12% |
| Unspecified | 1 | 6% |
| Other | 1 | 6% |
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| Unspecified | 1 | 6% |
| Social Sciences | 1 | 6% |
| Chemistry | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 7 | 41% |