Chapter title |
Genome Editing of Rat
|
---|---|
Chapter number | 9 |
Book title |
Genome Editing in Animals
|
Published in |
Methods in molecular biology, June 2017
|
DOI | 10.1007/978-1-4939-7128-2_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7127-5, 978-1-4939-7128-2
|
Authors |
Kaneko, Takehito, Takehito Kaneko |
Editors |
Izuho Hatada |
Abstract |
Many genetically engineered rat strains have been produced for biomedical research. The simple and quick production of knock-out and knock-in rats is currently possible using genome editing techniques incorporating zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), or clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9. Genome-edited animals have been produced by the introduction of endonucleases into embryos using conventional microinjection and a new electroporation method named Technique for Animal Knockout system by Electroporation (TAKE). This chapter introduces the latest protocols for producing genetically engineered rats using genome editing. |
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