Chapter title |
Genome Editing in Mouse and Rat by Electroporation
|
---|---|
Chapter number | 7 |
Book title |
Genome Editing in Animals
|
Published in |
Methods in molecular biology, June 2017
|
DOI | 10.1007/978-1-4939-7128-2_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7127-5, 978-1-4939-7128-2
|
Authors |
Kaneko, Takehito, Takehito Kaneko |
Editors |
Izuho Hatada |
Abstract |
Many knock-out/knock-in mouse and rat strains have been produced by genome editing techniques using engineered endonucleases, including zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), or clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9. Microinjection of engineered endonucleases into pronuclear-stage embryos is required to produce genome-edited rodents and the development of easy, rapid, and high-efficiency methods that do not require special skills such as microinjection is needed. This chapter presents a new technique called Technique for Animal Knockout system by Electroporation (TAKE), which produces genome-edited rodents by direct introduction of engineered endonucleases into intact embryos using electroporation. |
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