Chapter title |
Genome Editing in Mouse Zygotes and Embryonic Stem Cells by Introducing SgRNA/Cas9 Expressing Plasmids
|
---|---|
Chapter number | 6 |
Book title |
Genome Editing in Animals
|
Published in |
Methods in molecular biology, June 2017
|
DOI | 10.1007/978-1-4939-7128-2_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7127-5, 978-1-4939-7128-2
|
Authors |
Taichi Noda, Asami Oji, Masahito Ikawa, Noda, Taichi, Oji, Asami, Ikawa, Masahito |
Editors |
Izuho Hatada |
Abstract |
In mammalian cells, genome editing with the single guide RNA (sgRNA)/Cas9 complex allows for high targeting efficiency within a relatively short time frame with the added benefits of being low cost and easy to design. sgRNA/Cas9-mediated editing in mouse zygotes has accelerated the analysis of gene functions and the generation of mouse models of human diseases. Despite the benefits, this method still suffers from several problems, such as mosaicism in the founder generation which complicates genotyping and phenotypical analyses, and the low efficiency of more complicated genome editing. Thus, we recently established the system for genome editing in embryonic stem (ES) cells and its application for chimeric analysis in mice. In this section, we introduce the procedure for genome editing in mouse zygotes and ES cells. |
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