Chapter title |
CRISPR/Cas9-Mediated Targeted Knockin of Exogenous Reporter Genes in Zebrafish
|
---|---|
Chapter number | 14 |
Book title |
Genome Editing in Animals
|
Published in |
Methods in molecular biology, June 2017
|
DOI | 10.1007/978-1-4939-7128-2_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7127-5, 978-1-4939-7128-2
|
Authors |
Atsuo Kawahara, Kawahara, Atsuo |
Editors |
Izuho Hatada |
Abstract |
Genome editing technologies such as ZFN, TALEN, and CRISPR/Cas9 efficiently induce DNA double-stranded breaks (DSBs) at a targeted genomic locus, often resulting in a frameshift-mediated target gene disruption. It remains difficult to perform targeted integration of exogenous genes by genome editing technologies. DSBs can be restored through DNA repair mechanisms, such as non-homologous end joining (NHEJ), microhomology-mediated end joining (MMEJ), and homologous recombination (HR). It is well known that HR facilitates homology-dependent integration of donor DNA template into a targeted locus. Recently, both NHEJ-mediated and MMEJ-mediated targeted integrations of exogenous genes have been developed in zebrafish. This chapter summarizes the application of CRISPR/Cas9-mediated knock-in technology in zebrafish. |
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Mendeley readers
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Researcher | 7 | 20% |
Student > Ph. D. Student | 5 | 14% |
Other | 4 | 11% |
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Psychology | 1 | 3% |
Other | 2 | 6% |
Unknown | 4 | 11% |