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Genome Editing in Animals

Overview of attention for book
Cover of 'Genome Editing in Animals'

Table of Contents

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    Book Overview
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    Chapter 1 Construction and Evaluation of Zinc Finger Nucleases
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    Chapter 2 Current Overview of TALEN Construction Systems
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    Chapter 3 CRISPR/Cas9
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    Chapter 4 Computational Prediction of CRISPR/Cas9 Target Sites Reveals Potential Off-Target Risks in Human and Mouse
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    Chapter 5 Genome Editing of Mouse by Cytoplasmic Injection
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    Chapter 6 Genome Editing in Mouse Zygotes and Embryonic Stem Cells by Introducing SgRNA/Cas9 Expressing Plasmids
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    Chapter 7 Genome Editing in Mouse and Rat by Electroporation
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    Chapter 8 Generation of Knock-in Mouse by Genome Editing
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    Chapter 9 Genome Editing of Rat
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    Chapter 10 Gene Targeting in Rabbits: Single-Step Generation of Knock-out Rabbits by Microinjection of CRISPR/Cas9 Plasmids
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    Chapter 11 Genome Editing of Pig
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    Chapter 12 Genome Editing of Monkey
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    Chapter 13 Genome Editing Mediated by Primordial Germ Cell in Chicken
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    Chapter 14 CRISPR/Cas9-Mediated Targeted Knockin of Exogenous Reporter Genes in Zebrafish
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    Chapter 15 Genome Editing of Medaka
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    Chapter 16 A Simple Protocol for Loss-of-Function Analysis in Xenopus tropicalis Founders Using the CRISPR-Cas System
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    Chapter 17 Genome Editing of Silkworms
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    Chapter 18 Genome Editing in the Cricket, Gryllus bimaculatus
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    Chapter 19 Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease
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    Chapter 20 Genome Editing of C. elegans
Attention for Chapter 5: Genome Editing of Mouse by Cytoplasmic Injection
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Chapter title
Genome Editing of Mouse by Cytoplasmic Injection
Chapter number 5
Book title
Genome Editing in Animals
Published in
Methods in molecular biology, June 2017
DOI 10.1007/978-1-4939-7128-2_5
Pubmed ID
Book ISBNs
978-1-4939-7127-5, 978-1-4939-7128-2
Authors

Takuro Horii, Izuho Hatada, Horii, Takuro, Hatada, Izuho

Editors

Izuho Hatada

Abstract

CRISPR/Cas enables rapid production of genome-edited animals. The Cas9/gRNA component can be introduced to fertilized eggs in several ways. Here, we provide an instructional guide for the generation of knockout mice by cytoplasmic injection using in vitro transcribed Cas9 and gRNA.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 33%
Student > Doctoral Student 2 22%
Student > Bachelor 1 11%
Professor > Associate Professor 1 11%
Unknown 2 22%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 33%
Neuroscience 1 11%
Medicine and Dentistry 1 11%
Engineering 1 11%
Unknown 3 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 July 2017.
All research outputs
#20,429,992
of 22,982,639 outputs
Outputs from Methods in molecular biology
#9,929
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Outputs of similar age
#275,504
of 316,289 outputs
Outputs of similar age from Methods in molecular biology
#218
of 280 outputs
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