| Chapter title |
Genome Editing of Rat
|
|---|---|
| Chapter number | 9 |
| Book title |
Genome Editing in Animals
|
| Published in |
Methods in molecular biology, June 2017
|
| DOI | 10.1007/978-1-4939-7128-2_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7127-5, 978-1-4939-7128-2
|
| Authors |
Kaneko, Takehito, Takehito Kaneko |
| Editors |
Izuho Hatada |
| Abstract |
Many genetically engineered rat strains have been produced for biomedical research. The simple and quick production of knock-out and knock-in rats is currently possible using genome editing techniques incorporating zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), or clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9. Genome-edited animals have been produced by the introduction of endonucleases into embryos using conventional microinjection and a new electroporation method named Technique for Animal Knockout system by Electroporation (TAKE). This chapter introduces the latest protocols for producing genetically engineered rats using genome editing. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 22 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 18% |
| Student > Ph. D. Student | 1 | 5% |
| Unknown | 17 | 77% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 14% |
| Biochemistry, Genetics and Molecular Biology | 1 | 5% |
| Immunology and Microbiology | 1 | 5% |
| Unknown | 17 | 77% |