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Sphingosine-1-Phosphate

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Cover of 'Sphingosine-1-Phosphate'

Table of Contents

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    Book Overview
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    Chapter 3 Sphingosine-1-Phosphate (S1P) Signaling in Neural Progenitors
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    Chapter 4 Maintenance of Human Embryonic Stem Cells by Sphingosine-1-Phosphate and Platelet-Derived Growth Factor
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    Chapter 5 Measuring Sphingosine-1-Phosphate/Protein Interactions with the Kinetic Exclusion Assay
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    Chapter 6 A Cleanup Method for Mass Spectrometric Analysis of Sphingosine- and Ceramide-1-Phosphate in Blood and Solid Tissue Using a Phosphate Capture Molecule
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    Chapter 23 3D Stacked Construct: A Novel Substitute for Corneal Tissue Engineering
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    Chapter 24 Analysis of S1P Receptor Expression by Uterine Immune Cells Using Standardized Multi-parametric Flow Cytometry
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    Chapter 25 A Rapid Fluorescence Assay for Measuring Sphingosine-1-Phosphate Transporter Activity in Erythrocytes
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    Chapter 26 S1P Synergizes with Wall Shear Stress and Other Angiogenic Factors to Induce Endothelial Cell Sprouting Responses
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    Chapter 41 An Improved Isoform-Selective Assay for Sphingosine Kinase 1 Activity
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    Chapter 42 Methods for Analyzing Sphingosine-1-Phosphate Signaling in Human and Mouse Primary Mast Cells
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    Chapter 43 Ceramide and S1P Signaling in Embryonic Stem Cell Differentiation
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    Chapter 44 Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples and Cell Lines
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    Chapter 51 In Vitro Methods to Study the Modulation of Migration and Invasion by Sphingosine-1-Phosphate
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    Chapter 55 Measurement of Lysophosphatidic Acid and Sphingosine-1-Phosphate by Liquid Chromatography-Coupled Electrospray Ionization Tandem Mass Spectrometry
Attention for Chapter 41: An Improved Isoform-Selective Assay for Sphingosine Kinase 1 Activity
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Chapter title
An Improved Isoform-Selective Assay for Sphingosine Kinase 1 Activity
Chapter number 41
Book title
Sphingosine-1-Phosphate
Published in
Methods in molecular biology, May 2017
DOI 10.1007/7651_2017_41
Pubmed ID
28540558
Book ISBNs
978-1-4939-7412-2, 978-1-4939-7413-9
Authors

Melissa R. Pitman, Lorena T. Davies, Stuart M. Pitson

Abstract

Sphingosine kinases (SK) are the sole enzymes responsible for the production of sphingosine 1-phosphate (S1P). S1P is a signaling molecule with a plethora of targets, acting as both a second messenger intracellularly and extracellularly via a family of cell surface G-protein-coupled S1P receptors. The two sphingosine kinases, SK1 and SK2, arise from different genes and have some distinct and overlapping cellular functions that are regulated in part by differential cellular localization, developmental expression, and catalytic properties. Here, we describe an improved method for selectively detecting SK1 activity in vitro and cell lysates via the use of the zwitterionic detergent CHAPS, which effectively inhibits SK2 activity and thus allows selective analysis of SK1 activity in a range of cell samples. The assay measures the production of (32)P-labeled S1P following the addition of exogenous sphingosine and [γ(32)P]ATP. The S1P product can be purified by Bligh-Dyer solvent extraction, separated by thin layer chromatography (TLC), and the radiolabeled S1P quantified by exposing the TLC plate to a storage phosphor screen. This sensitive, reproducible assay can be used to selectively detect SK1 activity in tissue, cell, and recombinant protein samples.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Professor 1 33%
Student > Bachelor 1 33%
Unknown 1 33%
Readers by discipline Count As %
Pharmacology, Toxicology and Pharmaceutical Science 1 33%
Agricultural and Biological Sciences 1 33%
Unknown 1 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 June 2017.
All research outputs
#18,555,330
of 22,981,247 outputs
Outputs from Methods in molecular biology
#7,946
of 13,148 outputs
Outputs of similar age
#239,018
of 313,676 outputs
Outputs of similar age from Methods in molecular biology
#167
of 261 outputs
Altmetric has tracked 22,981,247 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,148 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 313,676 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 261 others from the same source and published within six weeks on either side of this one. This one is in the 20th percentile – i.e., 20% of its contemporaries scored the same or lower than it.

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