| Chapter title |
Real-Time PCR to Identify Staphylococci and Assay for Virulence from Blood
|
|---|---|
| Chapter number | 12 |
| Book title |
Diagnostic Bacteriology
|
| Published in |
Methods in molecular biology, June 2017
|
| DOI | 10.1007/978-1-4939-7037-7_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7035-3, 978-1-4939-7037-7
|
| Authors |
Charles E. Okolie, Okolie, Charles E. |
| Editors |
Kimberly A. Bishop-Lilly |
| Abstract |
The genus Staphylococcus includes pathogenic and non-pathogenic facultative anaerobes. Due to the plethora of virulence factors encoded in its genome, the species Staphylococcus aureus is known to be the most pathogenic. S. aureus strains harboring genes encoding virulence and antibiotic resistance are of public health importance. In clinical samples, however, pathogenic S. aureus is often mixed with putatively less pathogenic coagulase-negative staphylococci (CoNS), both of which can harbor mecA, the genetic driver for staphylococcal methicillin-resistance. In this chapter, the detailed practical procedure for operating a real-time pentaplex PCR assay in blood cultures is described. The pentaplex real-time PCR assay simultaneously detects markers for the presence of bacteria (16S rRNA), coagulase-negative staphylococcus (cns), S. aureus (spa), Panton-Valentine leukocidin (pvl), and methicillin resistance (mecA). |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Lecturer > Senior Lecturer | 2 | 17% |
| Librarian | 1 | 8% |
| Other | 1 | 8% |
| Student > Bachelor | 1 | 8% |
| Student > Ph. D. Student | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 5 | 42% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 2 | 17% |
| Biochemistry, Genetics and Molecular Biology | 2 | 17% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 8% |
| Social Sciences | 1 | 8% |
| Immunology and Microbiology | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 5 | 42% |